Home LiteratureArticle Details
PMID: 8035786 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of the transcription factor PU.1/Spi-1 in myeloid cell-restricted expression of an interferon-inducible gene encoding the human high-affinity Fc gamma receptor.

Molecular and cellular biology ·Vol. 14 ·No. 8 ·1994-08-00 ·Pages 5023-31

Perez C, Coeffier E, Moreau-Gachelin F, Wietzerbin J, Benech PD

Abstract

Induction by gamma interferon (IFN-gamma) of the gene encoding the human high-affinity Fc gamma receptor (Fc gamma R1) in myeloid cells requires an IFN-gamma response region (GRR) and a myeloid cell-activating transcription element (MATE). GRR and MATE interact with factors to form, respectively, an IFN-gamma-activating complex (GIRE-BP), depending on the phosphorylation of the 91-kDa protein (subunit of ISGF3), and a cell-type-specific complex (MATE-BP). Although GIRE-BP is detected in cells of different origins after IFN-gamma treatment, the presence of MATE-BP was found to be restricted to B- and myeloid cell lines. Sequence analysis of a cDNA encoding a polypeptide recognizing specifically the MATE motif led to the identification of this product as the proto-oncogene PU.1/Spi-1, a transcriptional activator expressed in myeloid and B cells. Expression of this factor in nonhematopoietic cells allowed IFN-gamma-induced expression of a reporter gene under control of the GRR and MATE sequences. The presence of these motifs in other gene promoters indicates that the binding of PU.1/Spi-1 and IFN regulatory proteins to their respective motifs could be part of a general mechanism leading to cell-type-restricted and IFN-induced gene expression.

MeSH Terms
B-Lymphocytes/metabolism Base Sequence Binding Sites Cell Line DNA-Binding Proteins/physiology Gene Expression Regulation Humans Interferon Type I/pharmacology Interferon-gamma/pharmacology Leukemia, Myeloid/metabolism Macromolecular Substances Molecular Sequence Data Promoter Regions, Genetic Proto-Oncogene Mas RNA, Messenger/genetics Receptors, IgG/genetics Recombinant Proteins Regulatory Sequences, Nucleic Acid Retroviridae Proteins, Oncogenic
Chemicals
DNA-Binding Proteins Interferon Type I MAS1 protein, human Macromolecular Substances Proto-Oncogene Mas RNA, Messenger Receptors, IgG Recombinant Proteins Retroviridae Proteins, Oncogenic v-Spi-1 protein, Friend spleen focus-forming virus Interferon-gamma
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Perez C
Unité 365 INSERM, Institut Curie, Paris, France.
Coeffier E
Moreau-Gachelin F
Wietzerbin J
Benech P D
References (50)
50 references, click to expand
  1. Establishment and characterization of a new human eosinophilic leukemia cell line.
    Blood. 1985 Dec;66(6):1233-40 PMID: 2415185
  2. Tyrosine phosphorylated p91 binds to a single element in the ISGF2/IRF-1 promoter to mediate induction by IFN alpha and IFN gamma, and is likely to autoregulate the p91 gene.
    EMBO J. 1994 Jan 1;13(1):158-67 PMID: 8306959
  3. Footprinting DNA-protein complexes in situ following gel retardation assays using 1,10-phenanthroline-copper ion: Escherichia coli RNA polymerase-lac promoter complexes.
    Biochemistry. 1987 Nov 17;26(23):7234-8 PMID: 3322397
  4. Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA.
    Cell. 1988 Feb 12;52(3):415-23 PMID: 2964277
  5. Characterization of a new megakaryocytic cell line: the Dami cell.
    Blood. 1988 Dec;72(6):1968-77 PMID: 3196874
  6. Isolation and expression of functional high-affinity Fc receptor complementary DNAs.
    Science. 1989 Jan 20;243(4889):378-81 PMID: 2911749
  7. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
    Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
  8. Interactions of alpha- and gamma-interferon in the transcriptional regulation of the gene encoding a guanylate-binding protein.
    EMBO J. 1989 Jul;8(7):2009-14 PMID: 2507314
  9. Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.
    Mol Cell Biol. 1989 Nov;9(11):4605-12 PMID: 2513475
  10. Cytoplasmic activation of ISGF3, the positive regulator of interferon-alpha-stimulated transcription, reconstituted in vitro.
    Genes Dev. 1989 Sep;3(9):1362-71 PMID: 2606351
  11. Structure and evolution of a human erythroid transcription factor.
    Nature. 1990 Jan 4;343(6253):92-6 PMID: 2104960
  12. The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene.
    Cell. 1990 Apr 6;61(1):113-24 PMID: 2180582
  13. The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors.
    Oncogene. 1990 May;5(5):663-8 PMID: 1693183
  14. The PU.1 transcription factor is the product of the putative oncogene Spi-1.
    Cell. 1990 Jun 29;61(7):1165-6 PMID: 2364426
  15. ISGF3, the transcriptional activator induced by interferon alpha, consists of multiple interacting polypeptide chains.
    Proc Natl Acad Sci U S A. 1990 Nov;87(21):8555-9 PMID: 2236065
  16. Interferon-alpha regulates nuclear translocation and DNA-binding affinity of ISGF3, a multimeric transcriptional activator.
    Genes Dev. 1990 Oct;4(10):1753-65 PMID: 2249773
  17. 5' regulatory region of a novel cytokine gene mediates selective activation by interferon gamma.
    J Exp Med. 1991 Feb 1;173(2):417-22 PMID: 1899103
  18. Cytoplasmic activation of GAF, an IFN-gamma-regulated DNA-binding factor.
    EMBO J. 1991 Apr;10(4):927-32 PMID: 1901265
  19. Isolation and characterization of a new mutant human cell line unresponsive to alpha and beta interferons.
    Mol Cell Biol. 1991 Aug;11(8):4189-95 PMID: 1906577
  20. A retinoic acid-responsive human zinc finger gene, MZF-1, preferentially expressed in myeloid cells.
    J Biol Chem. 1991 Aug 5;266(22):14183-7 PMID: 1860835
  21. Characterization of the promoter of the human gene encoding the high-affinity IgG receptor: transcriptional induction by gamma-interferon is mediated through common DNA response elements.
    Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11305-9 PMID: 1837149
  22. Characterization of the myeloid-specific CD11b promoter.
    Blood. 1992 Feb 15;79(4):865-70 PMID: 1346576
  23. Constitutive monocyte-restricted activity of NF-M, a nuclear factor that binds to a C/EBP motif.
    J Immunol. 1992 Jul 1;149(1):237-43 PMID: 1607656
  24. A protein tyrosine kinase in the interferon alpha/beta signaling pathway.
    Cell. 1992 Jul 24;70(2):313-22 PMID: 1386289
  25. Interferon-dependent signaling pathways: DNA elements, transcription factors, mutations, and effects of viral proteins.
    J Interferon Res. 1992 Jun;12(3):147-51 PMID: 1379292
  26. Interferon-dependent tyrosine phosphorylation of a latent cytoplasmic transcription factor.
    Science. 1992 Aug 7;257(5071):809-13 PMID: 1496401
  27. Definition of interferon gamma-response elements in a novel human Fc gamma receptor gene (Fc gamma RIb) and characterization of the gene structure.
    J Exp Med. 1992 Oct 1;176(4):1115-23 PMID: 1402657
  28. Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1.
    Mol Cell Biol. 1992 Oct;12(10):4297-304 PMID: 1406622
  29. Interferon gamma rapidly induces in human monocytes a DNA-binding factor that recognizes the gamma response region within the promoter of the gene for the high-affinity Fc gamma receptor.
    Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11964-8 PMID: 1334553
  30. Activation of transcription by IFN-gamma: tyrosine phosphorylation of a 91-kD DNA binding protein.
    Science. 1992 Dec 11;258(5089):1808-12 PMID: 1281555
  31. The activation domain of transcription factor PU.1 binds the retinoblastoma (RB) protein and the transcription factor TFIID in vitro: RB shows sequence similarity to TFIID and TFIIB.
    Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1580-4 PMID: 8434021
  32. The proto-oncogene PU.1 regulates expression of the myeloid-specific CD11b promoter.
    J Biol Chem. 1993 Mar 5;268(7):5014-20 PMID: 8095266
  33. Two cis-DNA elements involved in myeloid-cell-specific expression and gamma interferon (IFN-gamma) activation of the human high-affinity Fc gamma receptor gene: a novel IFN regulatory mechanism.
    Mol Cell Biol. 1993 Apr;13(4):2182-92 PMID: 8455606
  34. Effect of PU.1 phosphorylation on interaction with NF-EM5 and transcriptional activation.
    Science. 1993 Mar 12;259(5101):1622-5 PMID: 8456286
  35. Interferon gamma-induced transcription of the high-affinity Fc receptor for IgG requires assembly of a complex that includes the 91-kDa subunit of transcription factor ISGF3.
    Proc Natl Acad Sci U S A. 1993 May 1;90(9):4314-8 PMID: 8483949
  36. The response of gamma interferon activation factor is under developmental control in cells of the macrophage lineage.
    Mol Cell Biol. 1993 Jun;13(6):3245-54 PMID: 8497250
  37. Identification of cell-specific and developmentally regulated nuclear factors that direct myeloid and lymphoid expression of the CD11a gene.
    Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5364-8 PMID: 8099450
  38. Regulation of lymphoid-specific immunoglobulin mu heavy chain gene enhancer by ETS-domain proteins.
    Science. 1993 Jul 2;261(5117):82-6 PMID: 8316859
  39. The genomic structure of the murine ICSBP gene reveals the presence of the gamma interferon-responsive element, to which an ISGF3 alpha subunit (or similar) molecule binds.
    Mol Cell Biol. 1993 Jul;13(7):3951-63 PMID: 8321202
  40. In vitro activation of a transcription factor by gamma interferon requires a membrane-associated tyrosine kinase and is mimicked by vanadate.
    Mol Cell Biol. 1993 Jul;13(7):3984-9 PMID: 8321205
  41. Tyrosine phosphorylation of DNA binding proteins by multiple cytokines.
    Science. 1993 Sep 24;261(5129):1730-3 PMID: 8378773
  42. Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter.
    Cell. 1993 Sep 24;74(6):1135-45 PMID: 8402883
  43. Ets-related protein PU.1 regulates expression of the immunoglobulin J-chain gene through a novel Ets-binding element.
    Genes Dev. 1993 Oct;7(10):2006-15 PMID: 8406004
  44. Cell adhesion markers are expressed by a stable human endothelial cell line transformed by the SV40 large T antigen under vimentin promoter control.
    J Cell Physiol. 1993 Oct;157(1):41-51 PMID: 8408241
  45. Complementation of a mutant cell line: central role of the 91 kDa polypeptide of ISGF3 in the interferon-alpha and -gamma signal transduction pathways.
    EMBO J. 1993 Nov;12(11):4221-8 PMID: 7693454
  46. The protein tyrosine kinase JAK1 complements defects in interferon-alpha/beta and -gamma signal transduction.
    Nature. 1993 Nov 11;366(6451):129-35 PMID: 8232552
  47. Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway.
    Nature. 1993 Nov 11;366(6451):166-70 PMID: 7901766
  48. Polypeptide signalling to the nucleus through tyrosine phosphorylation of Jak and Stat proteins.
    Nature. 1993 Dec 9;366(6455):580-3 PMID: 7504784
  49. The macrophage transcription factor PU.1 directs tissue-specific expression of the macrophage colony-stimulating factor receptor.
    Mol Cell Biol. 1994 Jan;14(1):373-81 PMID: 8264604
  50. Spi-1 is a putative oncogene in virally induced murine erythroleukaemias.
    Nature. 1988 Jan 21;331(6153):277-80 PMID: 2827041
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.8.5023-5031.1994
Published
1994-08-00
Pages
5023-31
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359021
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.8.5023-5031.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2