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PMID: 8039513 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Telomerase activity in germline and embryonic cells of Xenopus.

The EMBO journal ·Vol. 13 ·No. 13 ·1994-07-01 ·Pages 3211-7

Mantell LL, Greider CW

Abstract

Telomerase is a ribonucleoprotein which synthesizes telomere repeats onto chromosome ends. Telomerase activity is involved in telomere length maintenance. We used Xenopus laevis as a model system to study the expression of telomerase activity in germline cells and during early development. We identified a non-processive telomerase activity in manually dissected nuclei of Xenopus stage VI oocytes. Telomerase activity was detected throughout oogenesis and embryogenesis. Telomerase was active in both S and M phase cell cycle extracts, suggesting that telomerase activity is not regulated with chromosomal DNA replication.

MeSH Terms
Animals Base Sequence Cell Nucleus/enzymology DNA Nucleotidylexotransferase/metabolism DNA Primers Female Male Mitosis Molecular Sequence Data Oogenesis Ovum/enzymology S Phase Spermatozoa/enzymology Telomere Tetrahymena Xenopus laevis
Chemicals
DNA Primers DNA Nucleotidylexotransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mantell L L
Cold Spring Harbor Laboratory, NY 11724.
Greider C W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-07-01
Pages
3211-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395213
Subset
IM
Grants
NIA NIH HHS · R01-AG-09383 · United States
External Links
PubMed source PMC Full Text View journal details
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