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PMID: 804169 Published · ppublish English Journal Article

Cell-free translation of immunoglobulin messenger RNA from MOPC-315 plasmacytoma and MOPC-315 NR, a variant synthesizing only light chain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 1 ·1975-01-00 ·Pages 224-8

Green M, Graves PN, Zehavi-Willner T, McInnes J, Pestka S

Abstract

Total poly(A)-containing mRNA was isolated from the MOPC-315 and MOPC-315 NR plasmacytomas. The RNA was further fractionated on sodium dodecyl sulfate-sucrose gradients. The MOPC-315 mRNA fractions directed the synthesis of both the heavy chain and light chain precursor of the MOPC-315 IgA protein in a cell-free extract of Ehrlich ascites tumor cells. None of the MOPC-315 NR mRNA fractions tested programmed the synthesis of the heavy chain in this system. Analysis of cell-free products by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by immunoprecipitation demonstrated that no translatable heavy chain mRNA could be extracted from the MOPC-315 NR variant plasmacytoma.

MeSH Terms
Animals Carcinoma, Ehrlich Tumor/metabolism Cell-Free System Electrophoresis, Polyacrylamide Gel Immunoglobulin A/biosynthesis Immunoglobulin Fragments Immunoglobulin Heavy Chains/biosynthesis Immunoglobulins/biosynthesis Methionine/metabolism Mice Myeloma Proteins/biosynthesis Neoplasms, Experimental/metabolism Plasmacytoma/metabolism Precipitin Tests Protein Biosynthesis RNA, Messenger/isolation & purification,metabolism RNA, Neoplasm/metabolism Sodium Dodecyl Sulfate Sulfur Radioisotopes
Chemicals
Immunoglobulin A Immunoglobulin Fragments Immunoglobulin Heavy Chains Immunoglobulins Myeloma Proteins RNA, Messenger RNA, Neoplasm Sulfur Radioisotopes Sodium Dodecyl Sulfate Methionine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Green M
Graves P N
Zehavi-Willner T
McInnes J
Pestka S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.1.224
Published
1975-01-00
Pages
224-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432275
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.1.224 PMC Full Text View journal details
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