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PMID: 8041713 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Yeast Srp1p has homology to armadillo/plakoglobin/beta-catenin and participates in apparently multiple nuclear functions including the maintenance of the nucleolar structure.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 15 ·1994-07-19 ·Pages 6880-4

Yano R, Oakes ML, Tabb MM, Nomura M

Abstract

SRP1, a suppressor of certain temperature-sensitive mutations in RNA polymerase I in Saccharomyces cerevisiae, encodes a protein that is associated with nuclear pores. By using a system of conditional SRP1 expression and by isolating temperature-sensitive srp1 mutants, we have demonstrated that Srp1p is essential for maintenance of the crescent-shaped nucleolar structure, RNA transcription, and the proper functions of microtubules as inferred from analysis of nuclear division/segregation and immunofluorescence microscopy of microtubules. Different mutant alleles showed significantly different phenotypes in relation to these apparently multiple functional roles of the protein. We have also found that eight imperfect 42-amino-acid tandem repeats present in Srp1p are similar to the 42-amino-acid repeats in armadillo/plakoglobin/beta-catenin proteins present in adhesive junction complexes of higher eukaryotes. We discuss this similarity in connection with the observed pleiotropic effects of srp1 mutations.

Related Genes

SRP1

MeSH Terms

Amino Acid Sequence Animals Armadillo Domain Proteins Cell Nucleolus/ultrastructure Cell Nucleus/physiology,ultrastructure Cytoskeletal Proteins/genetics Desmoplakins Drosophila Proteins Microtubules/metabolism Molecular Sequence Data Mutation Nuclear Proteins/genetics,physiology Proteins/genetics RNA Polymerase I/metabolism Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics,physiology,ultrastructure Sequence Homology, Amino Acid Trans-Activators Transcription, Genetic alpha Karyopherins beta Catenin gamma Catenin

Chemicals

Armadillo Domain Proteins Cytoskeletal Proteins Desmoplakins Drosophila Proteins Nuclear Proteins Proteins Trans-Activators alpha Karyopherins beta Catenin gamma Catenin RNA Polymerase I

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Yano R

Department of Biological Chemistry, University of California, Irvine 91717-1700.

Oakes M L

Tabb M M

Nomura M

References (21)

21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.91.15.6880

Published

1994-07-19

Pages

6880-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC44301

Subset

Grants

NIGMS NIH HHS · GM0713419 · United States

NIGMS NIH HHS · R37GM35949 · United States

External Links

PubMed source DOI: 10.1073/pnas.91.15.6880 PMC Full Text View journal details

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Yeast Srp1p has homology to armadillo/plakoglobin/beta-catenin and participates in apparently multiple nuclear functions including the maintenance of the nucleolar structure.

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