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PMID: 8045897 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The DNA replication fork blocked at the Ter site may be an entrance for the RecBCD enzyme into duplex DNA.

Journal of bacteriology ·Vol. 176 ·No. 15 ·1994-08-00 ·Pages 4656-63

Horiuchi T, Fujimura Y, Nishitani H, Kobayashi T, Hidaka M

Abstract

In Escherichia coli, eight kinds of chromosome-derived DNA fragments (named Hot DNA) were found to exhibit homologous recombinational hotspot activity, with the following properties. (i) The Hot activities of all Hot DNAs were enhanced extensively under RNase H-defective (rnh) conditions. (ii) Seven Hot DNAs were clustered at the DNA replication terminus region on the E. coli chromosome and had Chi activities (H. Nishitani, M. Hidaka, and T. Horiuchi, Mol. Gen. Genet. 240:307-314, 1993). Hot activities of HotA, -B, and -C, the locations of which were close to three DNA replication terminus sites, the TerB, -A, and -C sites, respectively, disappeared when terminus-binding (Tau or Tus) protein was defective, thereby suggesting that their Hot activities are termination event dependent. Other Hot groups showed termination-independent Hot activities. In addition, at least HotA activity proved to be dependent on a Chi sequence, because mutational destruction of the Chi sequence on the HotA DNA fragment resulted in disappearance of the HotA activity. The HotA activity which had disappeared was reactivated by insertion of a new, properly oriented Chi sequence at the position between the HotA DNA and the TerB site. On the basis of these observations and positional and orientational relationships between the Chi and the Ter sequences, we propose a model in which the DNA replication fork blocked at the Ter site provides an entrance for the RecBCD enzyme into duplex DNA.

MeSH Terms
Bacterial Proteins/metabolism Base Sequence Chromosomes, Bacterial DNA Helicases/metabolism DNA Replication DNA, Bacterial/biosynthesis DNA, Circular Escherichia coli/genetics Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/metabolism Models, Genetic Molecular Sequence Data Nucleic Acid Conformation Recombination, Genetic tau Proteins/metabolism
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Circular Escherichia coli Proteins tau Proteins tus protein, Bacteria tus protein, E coli Exodeoxyribonucleases Exodeoxyribonuclease V DNA Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Horiuchi T
National Institute for Basic Biology, Kyushu University, Fukuoka, Japan.
Fujimura Y
Nishitani H
Kobayashi T
Hidaka M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.15.4656-4663.1994
Published
1994-08-00
Pages
4656-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196287
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.15.4656-4663.1994 PMC Full Text View journal details
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