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PMID: 805418 Published · ppublish English Journal Article

Identification and separation of components of calf thymus DNA using a CsC1-netropsin density gradient.

Nucleic acids research ·Vol. 2 ·No. 3 ·1975-03-00 ·Pages 431-46

Votavová H, Sponar J

Abstract

Calf thymus DNA containing satellite components of various densities was used as a model to study the effect of netropsin on the density of DNA in a CsCl gradient. The binding of netropsin resulted in a decrease in density which depended upon the quantity of netropsin added and on the average composition of the DNA. Differences in density of DNA components were higher in CsCl - netropsin gradients than in simple CsCl gradients. By use of netropsin a main band and four satellite bands could be differentiated in calf thymus DNA. Satellite DNA's were isolated using preparativeCsCl - netropsin gradient centrifugation and were characterised by density and homogeneity in native and in reassociated state. Two of the satellite components, with densities of 1.722 and 1.714 g/cm minus 3, are probably of homogenous sequence, the other two components of densities 1.709 and 1.705 g/cm minus 3 appear to be heterogeneous.

MeSH Terms
Animals Anti-Bacterial Agents Binding Sites Cattle Centrifugation, Density Gradient Cesium DNA/isolation & purification Drosophila melanogaster/analysis Methods Peptides Protein Binding Thymus Gland/analysis
Chemicals
Anti-Bacterial Agents Peptides Cesium DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Votavová H
Sponar J
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/2.3.431
Published
1975-03-00
Pages
431-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342848
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/2.3.431 PMC Full Text View journal details
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