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PMID: 8061316 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Molecular cloning of a chloroplastic protein associated with both the envelope and thylakoid membranes.

Plant molecular biology ·Vol. 25 ·No. 4 ·1994-07-00 ·Pages 619-32

Li HM, Kaneko Y, Keegstra K

Abstract

Chloroplasts consist of six morphologically distinct compartments. Each compartment has a specific set of polypeptides that perform distinct biochemical functions. We report here the identification of a membrane-associated protein with a novel localization. This protein was synthesized as a 37 kDa precursor and was processed to a mature protein of 30 kDa after being imported into isolated pea chloroplasts. Fractionation of chloroplasts showed that the 30 kDa mature protein was associated with both of the envelope membranes as well as with thylakoid membranes. Immunocyto-chemical localization of the 30 kDa protein revealed that the protein occurred in clusters in the vicinity of both the envelope and the thylakoid. Possible functions of this 30 kDa protein, inferred from its novel localization pattern, are discussed.

MeSH Terms
Amino Acid Sequence Base Sequence Chloroplasts/metabolism Cloning, Molecular/methods Electrophoresis, Polyacrylamide Gel Fabaceae/cytology,metabolism,ultrastructure Immunohistochemistry Intracellular Membranes/metabolism Kinetics Molecular Sequence Data Molecular Weight Plant Proteins/analysis,biosynthesis,isolation & purification Plants, Medicinal
Chemicals
Plant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Li H M
Department of Botany, University of Wisconsin, Madison 53706.
Kaneko Y
Keegstra K
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29 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00029601
Published
1994-07-00
Pages
619-32
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
M73744
External Links
PubMed source DOI: 10.1007/BF00029601 View journal details
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