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PMID: 8061523 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 alpha.

The Plant cell ·Vol. 6 ·No. 6 ·1994-06-00 ·Pages 893-905

Durso NA, Cyr RJ

Abstract

The microtubules (MTs) of higher plant cells are organized into arrays with essential functions in plant cell growth and differentiation; however, molecular mechanisms underlying the organization and regulation of these arrays remain largely unknown. We have approached this problem using tubulin affinity chromatography to isolate carrot proteins that interact with MTs. From these proteins, a 50-kD polypeptide was selectively purified by exploiting its Ca(2+)-dependent binding to calmodulin (CaM). This polypeptide was identified as a homolog of elongation factor-1 alpha (EF-1 alpha)--a highly conserved and ubiquitous protein translation factor. The carrot EF-1 alpha homolog bundles MTs in vitro, and moreover, this bundling is modulated by the addition of Ca2+ and CaM together (Ca2+/CaM). A direct binding between the EF-1 alpha homolog and MTs was demonstrated, providing novel evidence for such an interaction. Based on these findings, and others discussed herein, we propose that an EF-1 alpha homolog mediates the lateral association of MTs in plant cells by a Ca2+/CaM-sensitive mechanism.

MeSH Terms
Amino Acid Sequence Animals Binding Sites Calcium/metabolism Calmodulin/metabolism Cattle Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Humans Microtubules/metabolism Molecular Sequence Data Multigene Family Peptide Elongation Factor 1 Peptide Elongation Factors/genetics Plant Development Plant Proteins, Dietary/metabolism Plants/genetics Sequence Homology, Amino Acid Tubulin/metabolism
Chemicals
Calmodulin Peptide Elongation Factor 1 Peptide Elongation Factors Plant Proteins, Dietary Tubulin Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Durso N A
Department of Biology, Pennsylvania State University, University Park 16802.
Cyr R J
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.6.6.893
Published
1994-06-00
Pages
893-905
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160487
Subset
IM
Databases
GENBANK
X60302
External Links
PubMed source DOI: 10.1105/tpc.6.6.893 PMC Full Text View journal details
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