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PMID: 8065949 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A rapid PCR fidelity assay.

Nucleic acids research ·Vol. 22 ·No. 15 ·1994-08-11 ·Pages 3259-60

Flaman JM, Frebourg T, Moreau V, Charbonnier F, Martin C, Ishioka C, Friend SH, Iggo R

Abstract

暂无摘要

MeSH Terms
DNA/biosynthesis DNA-Directed DNA Polymerase/metabolism Genes, p53 Mutation Polymerase Chain Reaction/methods Saccharomyces cerevisiae/genetics Taq Polymerase
Chemicals
DNA Deep Vent DNA polymerase Pfu DNA polymerase Taq Polymerase UlTma DNA polymerase DNA-Directed DNA Polymerase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Flaman J M
Unité de Génétique Moléculaire, CHU de Rouen, France.
Frebourg T
Moreau V
Charbonnier F
Martin C
Ishioka C
Friend S H
Iggo R
References (6)
6 references, click to expand
  1. The DNA-binding domain of p53 contains the four conserved regions and the major mutation hot spots.
    Genes Dev. 1993 Dec;7(12B):2556-64 PMID: 8276238
  2. Amplimers with 3'-terminal phosphorothioate linkages resist degradation by vent polymerase and reduce Taq polymerase mispriming.
    PCR Methods Appl. 1992 Nov;2(2):131-6 PMID: 1335814
  3. High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.
    Gene. 1991 Dec 1;108(1):1-6 PMID: 1761218
  4. Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity.
    Nucleic Acids Res. 1992 Jul 25;20(14):3551-4 PMID: 1641322
  5. Suppression of human colorectal carcinoma cell growth by wild-type p53.
    Science. 1990 Aug 24;249(4971):912-5 PMID: 2144057
  6. Screening patients for heterozygous p53 mutations using a functional assay in yeast.
    Nat Genet. 1993 Oct;5(2):124-9 PMID: 8252037
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.15.3259
Published
1994-08-11
Pages
3259-60
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310311
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/22.15.3259 PMC Full Text View journal details
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