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PMID: 8076591 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Solution structure of the LexA repressor DNA binding domain determined by 1H NMR spectroscopy.

The EMBO journal ·Vol. 13 ·No. 17 ·1994-09-01 ·Pages 3936-44

Fogh RH, Ottleben G, Rüterjans H, Schnarr M, Boelens R, Kaptein R

Abstract

The structure of the 84 residue DNA binding domain of the Escherichia coli LexA repressor has been determined from NMR data using distance geometry and restrained molecular dynamics. The assignment of the 1H NMR spectrum of the molecule, derived from 2- and 3-D homonuclear experiments, is also reported. A total of 613 non-redundant distance restraints were used to give a final family of 28 structures. The structured region of the molecule consisted of residues 4-69 and yielded a r.m.s. deviation from an average of 0.9 A for backbone and 1.6 A for all heavy atoms. The structure contains three regular alpha-helices at residues 6-21 (I), 28-35 (II) and 41-52 (III), and an antiparallel beta-sheet at residues 56-58 and 66-68. Helices II and III form a variant helix-turn-helix DNA binding motif, with an unusual one residue insert at residue 38. The topology of the LexA DNA binding domain is found to be the same as for the DNA binding domains of the catabolic activator protein, human histone 5, the HNF-3/fork head protein and the Kluyveromyces lactis heat shock transcription factor.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics Binding Sites Computer Simulation DNA/metabolism Escherichia coli/chemistry Helix-Loop-Helix Motifs Magnetic Resonance Spectroscopy Models, Molecular Molecular Sequence Data Mutation Peptide Fragments/chemistry Protein Conformation Repressor Proteins/chemistry Sequence Homology, Amino Acid Serine Endopeptidases Solutions/chemistry
Chemicals
Bacterial Proteins LexA protein, Bacteria Peptide Fragments Repressor Proteins Solutions DNA Serine Endopeptidases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fogh R H
Bijvoet Center for Biomolecular Research, Utrecht University, The Netherlands.
Ottleben G
Rüterjans H
Schnarr M
Boelens R
Kaptein R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-09-01
Pages
3936-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395313
Subset
IM
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