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PMID: 8089171 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulation of mitochondrial morphology and inheritance by Mdm10p, a protein of the mitochondrial outer membrane.

The Journal of cell biology ·Vol. 126 ·No. 6 ·1994-09-00 ·Pages 1361-73

Sogo LF, Yaffe MP

Abstract

Yeast cells with the mdm10 mutation possess giant spherical mitochondria and are defective for mitochondrial inheritance. The giant mitochondria display classical features of mitochondrial ultrastructure, yet they appear incapable of movement or division. Genetic analysis indicated that the mutant phenotypes resulted from a single nuclear mutation, and the isolated MDM10 gene restored wild-type mitochondrial distribution and morphology when introduced into mutant cells. MDM10 encodes a protein of 56.2 kD located in the mitochondrial outer membrane. Depletion of Mdm10p from cells led to a condensation of normally extended, tubular mitochondria into giant spheres, and reexpression of the protein resulted in a rapid restoration of normal mitochondrial morphology. These results demonstrate that Mdm10p can control mitochondrial morphology, and that it plays a role in the inheritance of mitochondria.

Related Genes
MDM10
MeSH Terms
Amino Acid Sequence Base Sequence Fungal Proteins/genetics,physiology Intracellular Membranes/chemistry Membrane Proteins/genetics,physiology Mitochondria/chemistry,ultrastructure Molecular Sequence Data Mutation Saccharomyces cerevisiae/genetics,ultrastructure Saccharomyces cerevisiae Proteins
Chemicals
Fungal Proteins MDM10 protein, S cerevisiae Membrane Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sogo L F
Department of Biology, University of California, San Diego, La Jolla 92093-0347.
Yaffe M P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.126.6.1361
Published
1994-09-00
Pages
1361-73
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2290945
Subset
IM
Grants
NIGMS NIH HHS · GM44614 · United States
Databases
GENBANK
X80874
External Links
PubMed source DOI: 10.1083/jcb.126.6.1361 PMC Full Text View journal details
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