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PMID: 8091213 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP.

Science (New York, N.Y.) ·Vol. 265 ·No. 5180 ·1994-09-23 ·Pages 1866-9

Crispino JD, Blencowe BJ, Sharp PA

Abstract

Individual small nuclear ribonucleoproteins (snRNPs) U1, U2, and U4/U6 were removed from nuclear extracts of HeLa cells by antisense affinity depletion. Addition of a highly purified preparation of SR proteins fully restored splicing activity in reactions depleted of U1 snRNP but did not reconstitute splicing in reactions depleted of the other snRNPs. Affinity selection experiments revealed that spliceosomes lacking U1 snRNA formed in the U1 snRNP-depleted reactions reconstituted with SR proteins. Thus, high concentrations of SR proteins facilitate the assembly of precursor messenger RNA (pre-mRNA) into a spliceosome in the absence of interactions with U1 snRNP.

MeSH Terms
Base Sequence Globins/genetics Molecular Sequence Data RNA Precursors/genetics RNA Splicing RNA, Small Nuclear/genetics,metabolism RNA-Binding Proteins/metabolism Ribonucleoprotein, U1 Small Nuclear/metabolism Ribonucleoprotein, U2 Small Nuclear/metabolism Spliceosomes/metabolism
Chemicals
RNA Precursors RNA, Small Nuclear RNA-Binding Proteins Ribonucleoprotein, U1 Small Nuclear Ribonucleoprotein, U2 Small Nuclear Globins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Crispino J D
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Blencowe B J
Sharp P A
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.8091213
Published
1994-09-23
Pages
1866-9
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Grants
NCI NIH HHS · P30 CA14051 · United States
NIAID NIH HHS · R01-AI32486 · United States
NIGMS NIH HHS · R01-GM34277 · United States
External Links
PubMed source DOI: 10.1126/science.8091213 View journal details
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