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PMID: 809424 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plasmid deoxyribonucleic acid in Bacillus subtilis and Bacillus pumilus.

Journal of bacteriology ·Vol. 124 ·No. 1 ·1975-10-00 ·Pages 484-90

Lovett PS, Bramucci MG

Abstract

Two of eighteen strains of Bacillus subtilis examined contained covalently closed circular duplex deoxyribonucleic acid (DNA) of homogeneous size and buoyant density. Strain ATCC 15841 contained about 16 copies per chromosome of plasmid pPL1, a circular DNA element having a molecular weight of about 4.7 times 10(6) and a buoyant density of 1.700. Strain ATCC 7003 contained about one to two copies per chromosome of plasmid pPL2. pPL2 had a molecular weight of about 46 times 10(6) and a buoyant density of 1.696. Strain ATCC 7003 appeared to be closely related to B. subtilis 168 by genetic, physiological, and biochemical criteria. Strain ATCC 15841 appeared to be much less closely related. B. pumilus ATCC 12140 contained two size classes of covalently closed circular duplex DNA. The plasmids pMB1 and pMB2 had molecular weights of about 6.8 times 10(6) and 5.3 times 10(6), respectively, and were present in several copies per chromosome.

MeSH Terms
Bacillus/analysis,classification Bacillus subtilis/analysis,classification DNA, Bacterial/analysis DNA, Circular/analysis Extrachromosomal Inheritance Molecular Weight Mutation Plasmids Transformation, Genetic
Chemicals
DNA, Bacterial DNA, Circular
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lovett P S
Bramucci M G
References (22)
22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.124.1.484-490.1975
Published
1975-10-00
Pages
484-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235917
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.124.1.484-490.1975 PMC Full Text View journal details
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