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PMID: 8096639 Published · ppublish English Journal Article Review

The molecular machinery for secretion is conserved from yeast to neurons.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 7 ·1993-04-01 ·Pages 2559-63

Bennett MK, Scheller RH

Abstract

A variety of approaches have been utilized to identify and characterize the molecules that mediate vesicular trafficking along the secretory pathway. Two approaches that have been particularly fruitful include the genetic dissection of the yeast secretory pathway and the biochemical characterization of proteins involved in the synaptic vesicle membrane trafficking in the mammalian nerve terminal. The recent convergence of these approaches suggests that common mechanisms may underlie a wide variety of vesicle-mediated transport steps. We discuss the results that support this possibility and propose a model for synaptic vesicle docking and fusion that incorporates evolutionarily conserved elements that may be part of a constitutive fusion machinery and specialized elements that may mediate regulatory events that are specific to the process of neurotransmitter release.

MeSH Terms
Animals Brain/physiology Cell Membrane/physiology Endocytosis Exocytosis Models, Biological Models, Neurological Nerve Tissue Proteins/physiology Neurons/metabolism,physiology Neurotransmitter Agents/physiology Saccharomyces cerevisiae/physiology Synaptic Vesicles/physiology
Chemicals
Nerve Tissue Proteins Neurotransmitter Agents
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bennett M K
Howard Hughes Medical Institute, Beckman Center, Department of Molecular and Cellular Physiology, Stanford University, CA 94305.
Scheller R H
References (56)
56 references, click to expand
  1. SV2, a brain synaptic vesicle protein homologous to bacterial transporters.
    Science. 1992 Aug 28;257(5074):1271-3 PMID: 1519064
  2. Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system.
    J Biol Chem. 1989 Jul 5;264(19):11061-4 PMID: 2472388
  3. Reversible phosphorylation--dephosphorylation determines the localization of rab4 during the cell cycle.
    EMBO J. 1992 Dec;11(12):4379-89 PMID: 1425574
  4. Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin.
    Nature. 1992 Oct 29;359(6398):832-5 PMID: 1331807
  5. Membrane fusion.
    Science. 1992 Nov 6;258(5084):917-24 PMID: 1439803
  6. The annexins and exocytosis.
    Science. 1992 Nov 6;258(5084):924-31 PMID: 1439804
  7. A role for synaptotagmin (p65) in regulated exocytosis.
    Cell. 1993 Jan 15;72(1):153-9 PMID: 8422678
  8. Brain contains two forms of synaptic vesicle protein 2.
    Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2150-4 PMID: 7681585
  9. ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway.
    Cell. 1990 Jun 29;61(7):1349-57 PMID: 2194670
  10. Proteinase mutants of Saccharomyces cerevisiae.
    Genetics. 1977 Jan;85(1):23-33 PMID: 320092
  11. Relationship between presynaptic calcium current and postsynaptic potential in squid giant synapse.
    Biophys J. 1981 Mar;33(3):323-51 PMID: 6261850
  12. Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells.
    J Cell Biol. 1985 Apr;100(4):1284-94 PMID: 2579958
  13. A 38,000-dalton membrane protein (p38) present in synaptic vesicles.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):4137-41 PMID: 3923488
  14. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles.
    Cell. 1985 Jul;41(3):1017-28 PMID: 3924408
  15. Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2.
    Nature. 1991 Apr 25;350(6320):715-8 PMID: 1902553
  16. Differential expression of the p65 gene family.
    Neuron. 1991 Jun;6(6):993-1007 PMID: 2054189
  17. Binding of synaptotagmin to the alpha-latrotoxin receptor implicates both in synaptic vesicle exocytosis.
    Nature. 1991 Sep 5;353(6339):65-8 PMID: 1881448
  18. Rapid activation of hippocampal casein kinase II during long-term potentiation.
    Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10232-6 PMID: 1946443
  19. Synaptic vesicle membrane proteins interact to form a multimeric complex.
    J Cell Biol. 1992 Feb;116(3):761-75 PMID: 1730776
  20. Cloning and sequencing of the yeast Saccharomyces cerevisiae SEC1 gene localized on chromosome IV.
    Yeast. 1991 Aug-Sep;7(6):643-50 PMID: 1767592
  21. Small GTP-binding proteins and their role in transport.
    Curr Opin Cell Biol. 1991 Aug;3(4):626-33 PMID: 1663370
  22. A family of proteins involved in intracellular transport.
    Cell. 1992 Jan 24;68(2):181-2 PMID: 1733497
  23. SNC1, a yeast homolog of the synaptic vesicle-associated membrane protein/synaptobrevin gene family: genetic interactions with the RAS and CAP genes.
    Proc Natl Acad Sci U S A. 1992 May 15;89(10):4338-42 PMID: 1316605
  24. Synaptotagmin: a calcium sensor on the synaptic vesicle surface.
    Science. 1992 May 15;256(5059):1021-5 PMID: 1589771
  25. Members of the VAMP family of synaptic vesicle proteins are components of glucose transporter-containing vesicles from rat adipocytes.
    J Biol Chem. 1992 Jun 15;267(17):11681-4 PMID: 1601842
  26. Neurexins: synaptic cell surface proteins related to the alpha-latrotoxin receptor and laminin.
    Science. 1992 Jul 3;257(5066):50-6 PMID: 1621094
  27. SEC22 and SLY2 are identical.
    Mol Cell Biol. 1992 Aug;12(8):3663-4 PMID: 1445580
  28. Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones.
    Science. 1992 Jul 10;257(5067):255-9 PMID: 1321498
  29. The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway.
    Cell. 1992 Sep 4;70(5):715-28 PMID: 1516130
  30. A novel 145 kd brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells.
    Cell. 1992 Sep 4;70(5):765-75 PMID: 1516133
  31. rab3 is a small GTP-binding protein exclusively localized to synaptic vesicles.
    Proc Natl Acad Sci U S A. 1990 Mar;87(5):1988-92 PMID: 2155429
  32. The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function.
    Mol Cell Biol. 1990 May;10(5):2214-23 PMID: 2183024
  33. Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C.
    Nature. 1990 May 17;345(6272):260-3 PMID: 2333096
  34. SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeast.
    Cell. 1990 May 18;61(4):709-21 PMID: 2111733
  35. BET1, BOS1, and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex.
    Mol Cell Biol. 1990 Jul;10(7):3405-14 PMID: 2192256
  36. Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments.
    Cell. 1990 Jul 27;62(2):317-29 PMID: 2115402
  37. Synaptophysin: a substrate for the protein tyrosine kinase pp60c-src in intact synaptic vesicles.
    Oncogene. 1990 Jul;5(7):1019-24 PMID: 1695724
  38. Synaptoporin, a novel putative channel protein of synaptic vesicles.
    Neuron. 1990 Oct;5(4):453-62 PMID: 2206533
  39. Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily.
    Mol Cell Biol. 1991 Feb;11(2):872-85 PMID: 1990290
  40. rab5 controls early endosome fusion in vitro.
    Cell. 1991 Mar 8;64(5):915-25 PMID: 1900457
  41. The synaptic vesicle protein SV2 is a novel type of transmembrane transporter.
    Cell. 1992 Sep 4;70(5):861-7 PMID: 1355409
  42. A ras-like protein is required for a post-Golgi event in yeast secretion.
    Cell. 1987 May 22;49(4):527-38 PMID: 3552249
  43. A synaptic vesicle protein with a novel cytoplasmic domain and four transmembrane regions.
    Science. 1987 Nov 20;238(4830):1142-4 PMID: 3120313
  44. Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA.
    EMBO J. 1987 Nov;6(11):3261-8 PMID: 3123215
  45. The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery.
    Cell. 1988 Mar 25;52(6):915-24 PMID: 3127057
  46. VAMP-1: a synaptic vesicle-associated integral membrane protein.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4538-42 PMID: 3380805
  47. Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack.
    Cell. 1988 Jul 15;54(2):221-7 PMID: 3390865
  48. Primary structure of the multifunctional alpha subunit protein of yeast fatty acid synthase derived from FAS2 gene sequence.
    J Biol Chem. 1988 Sep 5;263(25):12315-25 PMID: 2900835
  49. Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product.
    Mol Cell Biol. 1988 Oct;8(10):4098-109 PMID: 3054509
  50. Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):7852-6 PMID: 3186695
  51. Identification of synaptophysin as a hexameric channel protein of the synaptic vesicle membrane.
    Science. 1988 Nov 18;242(4881):1050-3 PMID: 2461586
  52. Molecular biology of synaptic vesicle-associated proteins.
    Trends Neurosci. 1988 Jun;11(6):241-2 PMID: 2465615
  53. Synaptobrevin: an integral membrane protein of 18,000 daltons present in small synaptic vesicles of rat brain.
    EMBO J. 1989 Feb;8(2):379-84 PMID: 2498078
  54. A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast.
    Nature. 1989 Jun 1;339(6223):355-9 PMID: 2657434
  55. Alpha-latrotoxin and related toxins.
    Pharmacol Ther. 1989;42(1):115-34 PMID: 2657806
  56. SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex.
    J Cell Biol. 1992 Nov;119(3):513-21 PMID: 1400588
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.7.2559
Published
1993-04-01
Pages
2559-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46134
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.7.2559 PMC Full Text View journal details
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