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PMID: 809768 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Absence of histone F1 in a mitotically dividing, genetically inactive nucleus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2672-6

Gorovsky MA, Keevert JB

Abstract

Histones were extracted from macro- and micronuclear chromatin of the ciliated protozoan Tetrahymena pyriformis. Conditions that resulted in macronuclear chromatin containing large amounts of histone F1 yielded micronuclear chromatin in which this histone was absent. Evidence is presented indicating that the absence of F1 from micronuclei is not a preparative artifact and that histone F1 is replaced by other histone fractions. Since micronuclei divide mitotically, while macronuclei divide amitotically, these results suggest that histone F1 and its phosphorylation do not play an indispensable role in the process of mitotic chromosome condensation, in chromosome replication, or in the separation of newly synthesized chromatids.

MeSH Terms
Animals Cell Nucleus/analysis,physiology Chromatin/analysis DNA/analysis Electrophoresis, Polyacrylamide Gel Histones/analysis Hot Temperature Mitosis Nucleic Acid Denaturation Tetrahymena pyriformis/analysis,physiology
Chemicals
Chromatin Histones DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gorovsky M A
Keevert J B
References (37)
37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.7.2672
Published
1975-07-00
Pages
2672-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432832
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.7.2672 PMC Full Text View journal details
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