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PMID: 8101525 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutations in human dynamin block an intermediate stage in coated vesicle formation.

The Journal of cell biology ·Vol. 122 ·No. 3 ·1993-08-00 ·Pages 553-63

van der Bliek AM, Redelmeier TE, Damke H, Tisdale EJ, Meyerowitz EM, Schmid SL

Abstract

The role of human dynamin in receptor-mediated endocytosis was investigated by transient expression of GTP-binding domain mutants in mammalian cells. Using assays which detect intermediates in coated vesicle formation, the dynamin mutants were found to block endocytosis at a stage after the initiation of coat assembly and preceding the sequestration of ligands into deeply invaginated coated pits. Membrane transport from the ER to the Golgi complex was unaffected indicating that dynamin mutants specifically block early events in endocytosis. These results demonstrate that mutations in the GTP-binding domain of dynamin block Tfn-endocytosis in mammalian cells and suggest that a functional dynamin GTPase is required for receptor-mediated endocytosis via clathrin-coated pits.

MeSH Terms
Alternative Splicing Amino Acid Sequence Ca(2+) Mg(2+)-ATPase/chemistry,genetics,physiology Dynamins Endocytosis Endoplasmic Reticulum/metabolism Endosomes/metabolism,ultrastructure Fluorescent Antibody Technique Golgi Apparatus/metabolism HeLa Cells Humans Molecular Sequence Data Mutation Transferrin/metabolism
Chemicals
Transferrin Ca(2+) Mg(2+)-ATPase Dynamins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
van der Bliek A M
Division of Biology, California Institute of Technology, Pasadena 91125.
Redelmeier T E
Damke H
Tisdale E J
Meyerowitz E M
Schmid S L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.122.3.553
Published
1993-08-00
Pages
553-63
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119674
Subset
IM
Grants
NCI NIH HHS · CA09270 · United States
NIGMS NIH HHS · GM40499 · United States
NIGMS NIH HHS · GM42445 · United States
Databases
GENBANK
L07807, L07808, L07809, L07810
External Links
PubMed source DOI: 10.1083/jcb.122.3.553 PMC Full Text View journal details
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