Home LiteratureArticle Details
PMID: 8103804 Published · ppublish English Comparative Study Journal Article

Congo red inhibition of scrapie agent replication.

Journal of virology ·Vol. 67 ·No. 10 ·1993-10-00 ·Pages 6270-2

Caughey B, Ernst D, Race RE

Abstract

Congo red inhibits the accumulation of protease-resistant PrP in scrapie-infected mouse neuroblastoma cells. Here we show that Congo red also inhibits the replication of scrapie infectivity in these cells. This observation is consistent with the idea that protease-resistant PrP is a vital component of the scrapie agent or that agent replication depends on the presence of protease-resistant PrP in the cell.

MeSH Terms
Animals Congo Red/toxicity Membrane Glycoproteins/biosynthesis Mice Neuroblastoma PrPSc Proteins Prions/biosynthesis,drug effects,isolation & purification Tumor Cells, Cultured Virus Replication/drug effects
Chemicals
Membrane Glycoproteins PrPSc Proteins Prions Congo Red
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Caughey B
Laboratory of Persistent Viral Diseases, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, Montana 59840.
Ernst D
Race R E
References (23)
23 references, click to expand
  1. Amyloid deposits and amyloidosis: the beta-fibrilloses (second of two parts).
    N Engl J Med. 1980 Jun 12;302(24):1333-43 PMID: 6990257
  2. Detection of proteinase K-resistant prion protein and infectivity in mouse spleen by 2 weeks after scrapie agent inoculation.
    J Gen Virol. 1992 Dec;73 ( Pt 12):3319-23 PMID: 1361522
  3. Identification of a protein that purifies with the scrapie prion.
    Science. 1982 Dec 24;218(4579):1309-11 PMID: 6815801
  4. A protease-resistant protein is a structural component of the scrapie prion.
    Cell. 1983 Nov;35(1):57-62 PMID: 6414721
  5. Scrapie infectivity, fibrils and low molecular weight protein.
    Nature. 1983 Dec 1-7;306(5942):476-8 PMID: 6685822
  6. Scrapie prions aggregate to form amyloid-like birefringent rods.
    Cell. 1983 Dec;35(2 Pt 1):349-58 PMID: 6418385
  7. The reticuloendothelial system in scrapie pathogenesis.
    J Gen Virol. 1984 Feb;65 ( Pt 2):423-8 PMID: 6198455
  8. Dextran sulphate 500 delays and prevents mouse scrapie by impairment of agent replication in spleen.
    J Gen Virol. 1984 Aug;65 ( Pt 8):1325-30 PMID: 6205119
  9. Antibodies to a scrapie prion protein.
    Nature. 1984 Aug 2-8;310(5976):418-21 PMID: 6431296
  10. A cellular gene encodes scrapie PrP 27-30 protein.
    Cell. 1985 Apr;40(4):735-46 PMID: 2859120
  11. Identification of scrapie prion protein-specific mRNA in scrapie-infected and uninfected brain.
    Nature. 1985 May 23-29;315(6017):331-3 PMID: 3923361
  12. Prolongation of scrapie incubation period by an injection of dextran sulphate 500 within the month before or after infection.
    J Gen Virol. 1986 Mar;67 ( Pt 3):463-73 PMID: 2419489
  13. Suppression of scrapie infection in mice by heteropolyanion 23, dextran sulfate, and some other polyanions.
    Antimicrob Agents Chemother. 1986 Sep;30(3):409-13 PMID: 2430521
  14. Antisera to scrapie-associated fibril protein and prion protein decorate scrapie-associated fibrils.
    J Virol. 1987 Jan;61(1):42-9 PMID: 2878092
  15. Analyses of frequency of infection, specific infectivity, and prion protein biosynthesis in scrapie-infected neuroblastoma cell clones.
    J Virol. 1988 Aug;62(8):2845-9 PMID: 2899175
  16. Protease sensitivity and nuclease resistance of the scrapie agent propagated in vitro in neuroblastoma cells.
    J Virol. 1991 Feb;65(2):1031-4 PMID: 1846182
  17. Chemoprophylaxis of scrapie in mice.
    J Gen Virol. 1991 Feb;72 ( Pt 2):457-60 PMID: 1704414
  18. Sulphate polyanions prolong the incubation period of scrapie-infected hamsters.
    J Gen Virol. 1992 Mar;73 ( Pt 3):661-5 PMID: 1372039
  19. Amphotericin B treatment dissociates in vivo replication of the scrapie agent from PrP accumulation.
    Nature. 1992 Apr 16;356(6370):598-601 PMID: 1348570
  20. The scrapie agent: "a virus by any other name".
    Curr Top Microbiol Immunol. 1991;172:195-232 PMID: 1687381
  21. Potent inhibition of scrapie-associated PrP accumulation by congo red.
    J Neurochem. 1992 Aug;59(2):768-71 PMID: 1352803
  22. Sulfated polyanion inhibition of scrapie-associated PrP accumulation in cultured cells.
    J Virol. 1993 Feb;67(2):643-50 PMID: 7678300
  23. Abnormal fibrils from scrapie-infected brain.
    Acta Neuropathol. 1981;54(1):63-74 PMID: 7195134
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.10.6270-6272.1993
Published
1993-10-00
Pages
6270-2
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC238051
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.67.10.6270-6272.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2