Abstract
Biogenesis of the type 1 pilus fiber in Escherichia coli requires the product of the fimC locus. We have demonstrated that FimC is a member of the periplasmic chaperone family. The deduced primary sequence of FimC shows a high degree of homology to PapD and fits well with the derived consensus sequence for periplasmic chaperones, predicting that it has an immunoglobulin-like topology. The chaperone activity of FimC was demonstrated by purifying a complex that FimC forms with the FimH adhesion. A fimC1 null allele could be complemented by the prototype member of the chaperone superfamily, PapD, resulting in the production of adhesive type 1 pili. The general mechanism of action of members of the chaperone superfamily was demonstrated by showing that the ability of PapD to assemble both P and type 1 pili was dependent on an invariant arginine residue (Arg-8), which forms part of a conserved subunit binding site in the cleft of PapD. We suggest that the conserved cleft is a subunit binding feature of all members of this protein family. These studies point out the general strategies used by Gram-negative bacteria to assemble adhesins into pilus fibers, allowing them to promote attachment to eukaryotic receptors.
MeSH Terms
Adhesins, Escherichia coli
Amino Acid Sequence
Bacterial Outer Membrane Proteins/chemistry,genetics,metabolism
Bacterial Proteins
Escherichia coli/genetics,physiology
Escherichia coli Proteins
Fimbriae Proteins
Fimbriae, Bacterial/physiology
Genes, Bacterial
Genetic Complementation Test
Hemagglutination Tests
Hemagglutinins/metabolism
Molecular Sequence Data
Plasmids
Protein Structure, Secondary
Sequence Homology, Amino Acid
Chemicals
Adhesins, Escherichia coli
Bacterial Outer Membrane Proteins
Bacterial Proteins
Escherichia coli Proteins
Hemagglutinins
fimC protein, E coli
Fimbriae Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jones C H
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110.
Pinkner J S
Nicholes A V
Slonim L N
Abraham S N
Hultgren S J
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