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PMID: 8105466 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Enhancement of peptide antigen presentation by a second peptide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 19 ·1993-10-01 ·Pages 8797-801

de Kroon AI, McConnell HM

Abstract

The influence of nonstimulatory "competitor" peptides on the binding of an antigenic peptide to a major histocompatibility complex (MHC) class II molecule was investigated. Using high-performance size-exclusion chromatography and fluorescein-labeled peptides, we show that the presence of the peptides dynorphin A-(1-13) and poly(L-lysine) results in enhancement rather than inhibition of the binding of hen egg lysozyme peptide-(107-116) [HEL-(107-116)] to the detergent-solubilized mouse class II molecule IEd. In parallel, dynorphin A-(1-13) and poly(L-lysine) were found to enhance the specific activation of an IEd-restricted T-cell hybridoma by HEL-(107-116). A molecular mechanism involving an intermediate two peptide-MHC class II protein complex is proposed to explain the enhancement of peptide binding to class II molecules by an irrelevant second peptide.

MeSH Terms
Amino Acid Sequence Animals Antigens/immunology,metabolism Binding, Competitive Cell Line Chickens Dyneins/pharmacology Dynorphins/pharmacology Histocompatibility Antigens Class II/isolation & purification,metabolism Hybridomas/immunology Lymphocyte Activation/drug effects Mice Molecular Sequence Data Muramidase/immunology,metabolism Peptide Fragments/pharmacology Peptides/chemical synthesis,chemistry,pharmacology Polylysine/pharmacology T-Lymphocytes/drug effects,immunology
Chemicals
Antigens Histocompatibility Antigens Class II Peptide Fragments Peptides Polylysine dynorphin (1-13) Dynorphins Muramidase Dyneins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
de Kroon A I
Department of Chemistry, Stanford University, CA 94305.
McConnell H M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.19.8797
Published
1993-10-01
Pages
8797-801
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47447
Subset
IM
Grants
NIAID NIH HHS · 5R37 AI13587-17 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.19.8797 PMC Full Text View journal details
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