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PMID: 810662 Published · ppublish English Journal Article

Ribosomal proteins from streptomycin-resistant and dependent mutants, and revertants from streptomycin-dependence to independence in Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 139 ·No. 4 ·1975-09-08 ·Pages 293-301

Ito T, Kosugi H, Higo K, Osawa S

Abstract

Streptomycin-resistant and dependent mutants were isolated from Bacillus subtilis ATCC 6633. Ribosomal proteins were analysed from six such mutants with chromatography on carboxymethyl cellulose or phosphocellulose columns. An altered specific 30s ribosomal protein, 30B, could be detected in all of these mutants. Streptomycin-independent revertants were isolated from a streptomycin-dependent strain. Some of them had an altered 30s ribosomal protein, 30A, and some others had an altered 30C protein. It was shown that from the data of partial N-terminal amino acid sequences together with amino acid compositions and mobilities on two-dimensional gel electrophoresis of these proteins that 30A, 30B, and 30C proteins were homologous with S5, S12 and S4 of E. coli 30s ribosomal proteins, respectively.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/analysis,drug effects Bacterial Proteins/analysis Chromatography Drug Resistance, Microbial Electrophoresis Mutation Ribosomal Proteins/analysis Streptomycin/pharmacology
Chemicals
Bacterial Proteins Ribosomal Proteins Streptomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ito T
Kosugi H
Higo K
Osawa S
References (28)
28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267969
Published
1975-09-08
Pages
293-301
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267969
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