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PMID: 8107774 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A brain serine/threonine protein kinase activated by Cdc42 and Rac1.

Nature ·Vol. 367 ·No. 6458 ·1994-01-06 ·Pages 40-6

Manser E, Leung T, Salihuddin H, Zhao ZS, Lim L

Abstract

A new brain serine/threonine protein kinase may be a target for the p21ras-related proteins Cdc42 and Rac1. The kinase sequence is related to that of the yeast protein STE20, implicated in pheromone-response pathways. The kinase complexes specifically with activated (GTP-bound) p21, inhibiting p21 GTPase activity and leading to kinase autophosphorylation and activation. Autophosphorylated kinase has a decreased affinity for Cdc42/Rac, freeing the p21 for further stimulatory activities or downregulation by GTPase-activating proteins. This bimolecular interaction provides a model for studying p21 regulation of mammalian phosphorylation signalling pathways.

MeSH Terms

Amino Acid Sequence Animals Brain/enzymology Cattle Enzyme Activation GTP Phosphohydrolases/antagonists & inhibitors GTP-Binding Proteins/metabolism Guanosine Diphosphate/metabolism Guanosine Triphosphate/metabolism Intracellular Signaling Peptides and Proteins MAP Kinase Kinase Kinases Molecular Sequence Data Phosphorylation Protein Binding Protein Serine-Threonine Kinases/metabolism Protein-Tyrosine Kinases/metabolism Rats Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Signal Transduction p21-Activated Kinases rac GTP-Binding Proteins rho GTP-Binding Proteins

Chemicals

Intracellular Signaling Peptides and Proteins Saccharomyces cerevisiae Proteins Guanosine Diphosphate Guanosine Triphosphate Protein-Tyrosine Kinases Protein Serine-Threonine Kinases p21-Activated Kinases MAP Kinase Kinase Kinases STE20 protein, S cerevisiae GTP Phosphohydrolases GTP-Binding Proteins rac GTP-Binding Proteins rho GTP-Binding Proteins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Manser E

Institute of Molecular & Cell Biology, National University of Singapore, Kent Ridge.

Leung T

Salihuddin H

Zhao Z S

Lim L

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/367040a0

Published

1994-01-06

Pages

40-6

Language

English

Region

England

NLM ID

0410462

Subset

Databases

GENBANK

U23443

External Links

PubMed source DOI: 10.1038/367040a0 View journal details

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A brain serine/threonine protein kinase activated by Cdc42 and Rac1.

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