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PMID: 810982 Published · ppublish ger Comparative Study English Abstract Journal Article

[A glucose dehydrogenase for the determination of glucose concentrations in body fluids (author's transl)].

Eine Glucose-Dehydrogenase für die Glucose-Bestimmung in Körperflüssigkeiten

Zeitschrift fur klinische Chemie und klinische Biochemie ·Vol. 13 ·No. 3 ·1975-03-00 ·Pages 101-7

Banauch D, Brümmer W, Ebeling W, Metz H, Rindfrey H, Lang H, Leybold K, Rick W, Staudinger HJ

Abstract

The isolation of glucose dehydrogenase from Bacillus megaterium M 1286 is outlined. Data on the specificity of the enzyme towards carbohydrates are given. A specific method for glucose determination using this enzyme was developed. Methods and results of four variants of this glucose determination are presented: End point determination in the UV range, determination with formazan as reaction product, kinetic determination in the UV range, and continuous flow analysis in the UV range (AutoAnalyzer method).

MeSH Terms
Alcohol Oxidoreductases/isolation & purification Autoanalysis Bacillus megaterium/enzymology Blood Glucose/analysis Carbohydrate Epimerases Colorimetry Glucose/analysis Humans Kinetics Methods NADH, NADPH Oxidoreductases Spectrophotometry, Ultraviolet
Chemicals
Blood Glucose Alcohol Oxidoreductases NADH, NADPH Oxidoreductases Carbohydrate Epimerases Glucose
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Banauch D
Brümmer W
Ebeling W
Metz H
Rindfrey H
Lang H
Leybold K
Rick W
Staudinger H J
Article Info
Journal
Zeitschrift fur klinische Chemie und klinische Biochemie
Abbr.
Z Klin Chem Klin Biochem
ISSN
0044-2933
Published
1975-03-00
Pages
101-7
Language
ger
Region
Germany
NLM ID
7607250
Subset
IM
External Links
PubMed source
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