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PMID: 8114719 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning, chromosomal location, and characterization of mouse E2F1.

Molecular and cellular biology ·Vol. 14 ·No. 3 ·1994-03-00 ·Pages 1861-9

Li Y, Slansky JE, Myers DJ, Drinkwater NR, Kaelin WG, Farnham PJ

Abstract

E2F has been implicated in growth control because of its association with the retinoblastoma protein and the presence of E2F binding sites in the promoters of several growth-regulated genes. Proteins that bind to an E2F site have been cloned from human and mouse cells. However, these two proteins (human E2F1 and mouse DP-1) are quite different in sequence. We have now cloned a mouse cDNA encoding a protein 86% identical to the human E2F1 protein. The mouse E2F1 cDNA encodes a 430-amino-acid protein with a predicted molecular weight of 46,322 and detects mRNAs of 2.7 and 2.2 kb. Using primers complementary to sequences in the mouse E2F1 3' untranslated region, we mapped the mouse E2F1 gene to chromosome 2, near the Agouti and c-src loci. To understand the role of the different E2F family members in the growth of mouse NIH 3T3 cells, we examined the levels of E2F1 and DP-1 mRNAs in different stages of the cell cycle. Since the levels of E2F1 but not DP-1 mRNA correlated with changes in transcription from the dhfr promoter, we examined whether E2F1 could activate various growth-regulated promoters. We found that E2F1 could activate some (dhfr, thymidine kinase, and DNA polymerase alpha) but not all (thymidylate synthase, cad, and c-myc) of these promoters. On the basis of changes in levels of E2F1 and its ability to transactivate growth-regulated promoters, we propose that E2F1 may mediate growth factor-initiated signal transduction.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Carrier Proteins Cell Cycle Cell Cycle Proteins Chromosome Mapping Cloning, Molecular DNA, Complementary/genetics DNA-Binding Proteins/genetics,metabolism E2F Transcription Factors E2F1 Transcription Factor Gene Expression Regulation Genes Mice Molecular Sequence Data Promoter Regions, Genetic RNA, Messenger/genetics Retinoblastoma-Binding Protein 1 Transcription Factor DP1 Transcription Factors/genetics Transcription, Genetic
Chemicals
Arid4a protein, mouse Carrier Proteins Cell Cycle Proteins DNA, Complementary DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor E2f1 protein, mouse RNA, Messenger Retinoblastoma-Binding Protein 1 Tfdp1 protein, mouse Transcription Factor DP1 Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Li Y
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Slansky J E
Myers D J
Drinkwater N R
Kaelin W G
Farnham P J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.3.1861-1869.1994
Published
1994-03-00
Pages
1861-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358544
Subset
IM
Grants
NCI NIH HHS · CA07175 · United States
NCI NIH HHS · CA23076 · United States
NCI NIH HHS · CA45240 · United States
Databases
GENBANK
L21973
External Links
PubMed source DOI: 10.1128/mcb.14.3.1861-1869.1994 PMC Full Text View journal details
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