Home LiteratureArticle Details
PMID: 812488 Published · ppublish English Journal Article

Plasma-membrane-associated immunoglobulins and other polypeptides of pig mesenteric node lymphocytes.

The Biochemical journal ·Vol. 148 ·No. 3 ·1975-06-00 ·Pages 417-23

Chavin SI, Johnson SM, Holliman A

Abstract

Plasma membranes from pig mesenteric lymph-node lymphocytes contain a large number of polypeptide chains ranging in molecular weight from 20,000 to greater than 470,000, with a major component of 46,000. There are approx. 12-15 glycoproteins. The membranes contain immunoglobulin G, which comprises 0.6% of the total protein. Immunoglobulin M is also detected, but has not been accurately quantified for technical reasons. Possible origins of the membrane-associated immunoglobulin are briefly discussed. It is concluded that the immunoglobulin is probably associated with the plasma membrane in the intact cell.

MeSH Terms
Animals Cell Membrane/immunology Electrophoresis, Polyacrylamide Gel Glycoproteins/analysis Immune Sera Immunodiffusion Immunoglobulin G/analysis Immunoglobulin M/analysis Lymph Nodes/immunology Lymphocytes/immunology Microscopy, Electron Molecular Weight Peptides/analysis Receptors, Antigen, B-Cell/analysis Sheep/immunology Swine
Chemicals
Glycoproteins Immune Sera Immunoglobulin G Immunoglobulin M Peptides Receptors, Antigen, B-Cell
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chavin S I
Johnson S M
Holliman A
References (40)
40 references, click to expand
  1. THE ISOLATION AND CHARACTERIZATION OF SUBCELLULAR COMPONENTS OF THE EPITHELIAL CELLS OF RABBIT SMALL INTESTINE.
    Biochem J. 1965 Jul;96:159-71 PMID: 14343125
  2. Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.
    J Biol Chem. 1971 Oct 25;246(20):6328-34 PMID: 5127429
  3. Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase.
    Biochem J. 1961 Sep;80:649-54 PMID: 13734134
  4. A simple method for the isolation and purification of total lipides from animal tissues.
    J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
  5. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  6. Lymphocyte plasma membranes: analysis of proteins and glycoproteins by SDS-gel electrophoresis.
    Blood. 1973 Jan;41(1):131-40 PMID: 4682075
  7. The osmotic insensitivity of sonicated liposomes and the density of phospholipid-cholesterol mixtures.
    Biochim Biophys Acta. 1973 Apr 25;307(1):20-6 PMID: 4711187
  8. Immunoglobulin determinants on lymphocytes of adult, neonatal and foetal pigs.
    Nat New Biol. 1972 Sep 27;239(91):114-6 PMID: 4507517
  9. Thymus-derived (T) cell immunoglobulins. Presence of a receptor site for IgG and absence of large amounts of "buried" Ig determinants on T cells.
    J Exp Med. 1972 Nov 1;136(5):1323-8 PMID: 4117193
  10. Biochemical and biological characteristics of lymphocyte surface immunoglobulin.
    Transplant Rev. 1973;14:3-49 PMID: 4125686
  11. Subcellular separation and molecular nature of human histocompatibility antigens (HL-A).
    Nature. 1974 Feb 15;247(5441):457-61 PMID: 4131934
  12. Common antigenic structures of HL-A antigens. II. Small fragments derived from papain-solubilized HL-A antigen molecules.
    Immunology. 1974 Jan;26(1):155-68 PMID: 4136153
  13. The control of humoral and associative antibody synthesis.
    Transplant Rev. 1972;11:217-67 PMID: 4129422
  14. The isolation and characterization of plasma membranes from normal and leukaemic cells of mice.
    Biochim Biophys Acta. 1973 Sep 27;323(1):55-68 PMID: 4148030
  15. Serum lipoprotein apoproteins as major protein constituents of the human erythrocyte membrane.
    Biochim Biophys Acta. 1974 Apr 11;342(2):213-28 PMID: 4364841
  16. The fluid mosaic model of the structure of cell membranes.
    Science. 1972 Feb 18;175(4023):720-31 PMID: 4333397
  17. Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis.
    Biochem Biophys Res Commun. 1971 Jul 16;44(2):390-5 PMID: 4334138
  18. Isolation and composition of human thymocyte plasma membrane.
    Biochim Biophys Acta. 1972 Jul 3;274(1):22-7 PMID: 4339743
  19. Subcellular fractionation of human lymphocytes. Isolation of two plasma membrane fractions and comparison of the protein components of the various lymphocytic organelles.
    Biochim Biophys Acta. 1973 Jan 2;291(1):93-106 PMID: 4346313
  20. An accurate and convenient organic phosphorus assay.
    Anal Biochem. 1971 Feb;39(2):527-30 PMID: 4324534
  21. Preparation and characterization of the plasma membrane of pig lymphocytes.
    Biochem J. 1970 Nov;120(1):133-43 PMID: 4321928
  22. Membrane-bound enzymes and membrane ultrastructure.
    Biochim Biophys Acta. 1973 Apr 3;300(1):1-30 PMID: 4269073
  23. The site of diphosphoinositide synthesis in rat liver.
    Biochem Biophys Res Commun. 1965 Nov 22;21(4):333-8 PMID: 4286175
  24. Novel human serum protein from fibroblast plasma membrane.
    Nature. 1974 Apr 26;248(5451):789-91 PMID: 4209027
  25. IgM in bone marrow-derived lymphocytes. Synthesis, surface deposition, turnover and carbohydrate composition in unstimulated mouse B cells.
    Eur J Immunol. 1974 Mar;4(3):170-80 PMID: 4604559
  26. A critical evaluation of plasma membrane fractionation.
    Biochim Biophys Acta. 1973 Nov;300(3):211-54 PMID: 4593831
  27. Cellular immunology in the pig.
    Proc R Soc Med. 1973 Dec;66(12):1155-60 PMID: 4591083
  28. Possible role for the Fc receptor on B lymphocytes.
    Nature. 1974 Jun 7;249(457):573-4 PMID: 4545851
  29. Synthesis, transport, dynamics and fate of cell surface Ig and alloantigens in murine lymphocytes.
    Transplant Rev. 1973;14:50-75 PMID: 4542308
  30. The binding of anti-immunoglobulin antibodies to rat thymocytes and thoracic duct lymphocytes.
    Eur J Immunol. 1974 Feb;4(2):91-7 PMID: 4546942
  31. Plasma membranes of bursal, thymic and circulating lymphocytes have unique proteins.
    Biochem Biophys Res Commun. 1973 Jan 4;50(1):118-26 PMID: 4539602
  32. Lymphocyte plasma membranes. I. Characterization of an antigenic plasma membrane constituent on rat thymus cells.
    J Immunol. 1974 Jan;112(1):249-59 PMID: 4812173
  33. Size and configuration of glycoprotein fragments cleaved from tumor cells by proteolysis.
    J Biol Chem. 1973 May 25;248(10):3405-10 PMID: 4702869
  34. Protein subunits: a table (revised edition).
    Arch Biochem Biophys. 1972 Mar;149(1):1-14 PMID: 5017251
  35. Purification and properties of HeLa cell plasma membranes.
    J Biol Chem. 1971 Aug 25;246(16):5162-75 PMID: 4936724
  36. Selective enzyme purification by affinity chromatography.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43 PMID: 4971842
  37. Polyacrylamide gel electrophoresis.
    Science. 1971 Apr 30;172(3982):440-51 PMID: 4927678
  38. Rosette formation with sheep erythrocytes--a possible T-cell marker in the pig.
    Int Arch Allergy Appl Immunol. 1975;48(2):261-75 PMID: 1090543
  39. Plasma membrane protein subunit composition. A comparative study by discontinuous electrophoresis in sodium dodecyl sulfate.
    J Biol Chem. 1971 Oct 25;246(20):6335-8 PMID: 5127430
  40. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS.
    J Lipid Res. 1964 Jan;5:126-7 PMID: 14173318
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1480417
Published
1975-06-00
Pages
417-23
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1165558
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1480417 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2