Determination of the primary structure of a mouse IgG2a immunoglobulin. Amino-acid sequence of the light chain.

European journal of biochemistry ·Vol. 59 ·No. 2 ·1975-11-15 ·Pages 525-37

Schiff C, Fougereau M

Abstract

The amino acid sequence of the light chain of the mouse monoclonal MOPC 173 immunoglobulin molecule (IgG2a,chi) is presented. This kappa chain contains 214 residues. Comparisons of this sequence with murine kappa chains already published by other workers bring a confirmation of the large size of the murine V chi chain pool. A complete identity was found with the constant region of the light chain of MOPC 21 from residue 97 to residue 214.

MeSH Terms

Amino Acid Sequence Amino Acids/analysis Cell Line Chymotrypsin Cyanogen Bromide Immunoglobulin G/analysis Immunoglobulin Light Chains/analysis Immunoglobulin kappa-Chains/analysis Myeloma Proteins/analysis Pepsin A Peptide Fragments/analysis Trypsin

Chemicals

Amino Acids Immunoglobulin G Immunoglobulin Light Chains Immunoglobulin kappa-Chains Myeloma Proteins Peptide Fragments Chymotrypsin Trypsin Pepsin A Cyanogen Bromide

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Schiff C

Fougereau M

Article Info

Journal

European journal of biochemistry

Abbr.

Eur J Biochem

ISSN

0014-2956

DOI

10.1111/j.1432-1033.1975.tb02479.x

Published

1975-11-15

Pages

525-37

Language

English

Region

England

NLM ID

0107600

Subset

External Links

PubMed source DOI: 10.1111/j.1432-1033.1975.tb02479.x

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Determination of the primary structure of a mouse IgG2a immunoglobulin. Amino-acid sequence of the light chain.

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