Fine genetic structure of the 2D3-2F5 region of the X-chromosome of Drosophila melanogaster.

Molecular & general genetics : MGG ·Vol. 141 ·No. 3 ·1975-12-01 ·Pages 269-75

Gvozdev VA, Gostimsky SA, Gerasimova TI, Dubrovskaya ES, Braslavskaya OY

Abstract

97 lethal and semilethal mutations were induced by ethyl methanesulfonate, nitrosomethyl urea and gamma-irradiation in the 2D3-F5 region of the X-chromosome of D. melanogaster. Approximately 1 per cent of the tested X-chromosomes carried a lethal in the 2D3-2F5 region. The mutation frequencies per band or DNA content in the region and the whole X-chromosome are equal. Complementation analysis revealed at least 10 functionally independent essential loci in this region including about 10 bands. The data presented in this study support the one band--one gene hypothesis. The Pgd locus coding for 6-phosphogluconate dehydrogenase (6PGD) is mapped in the 2D3 (OR 2D4) band. Isolation of 11 lethal or semilethal point mutations with null or reduced 6PGD activity shows that the Pgd locus is a vital one.

MeSH Terms

Chromosome Mapping Drosophila melanogaster/metabolism Ethyl Methanesulfonate/pharmacology Gamma Rays Genes Genes, Lethal Methylnitrosourea/pharmacology Mutagens Mutation Phosphogluconate Dehydrogenase Radiation Genetics Sex Chromosomes/drug effects,radiation effects

Chemicals

Mutagens Methylnitrosourea Ethyl Methanesulfonate Phosphogluconate Dehydrogenase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Gvozdev V A

Gostimsky S A

Gerasimova T I

Dubrovskaya E S

Braslavskaya O Y

References (15)

15 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00341805

Published

1975-12-01

Pages

269-75

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00341805

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Fine genetic structure of the 2D3-2F5 region of the X-chromosome of Drosophila melanogaster.

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