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PMID: 8132220 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vitro modulation of interleukin-1 beta secretion by cultured rat doxorubicin-stimulated whole glomeruli and dissociated mesangial glomerular cells.

Immunology ·Vol. 81 ·No. 1 ·1994-01-00 ·Pages 53-7

Bricio T, Molina A, Martin A, Mampaso F

Abstract

Doxorubicin-stimulated whole rat glomeruli and dissociated mesangial and resident glomerular macrophage cells produced the release of interleukin (IL)-1 beta cytokine. This activity increased after the addition of lipopolysaccharide (LPS) or LPS plus indomethacin to the cultures. In the presence of WEB2086 [platelet-activating factor (PAF)-acether antagonist], this activity showed a drastic reduction, without modification after sodium furegrelate (thromboxane synthetase inhibitor) was added to the cultures. Our results also demonstrate that this IL-1 beta activity is mainly produced by glomerular-resident macrophage cells. These findings support the important role by both IL-1 beta and PAF-acether mediator factors, at the cellular level, in the rat model of doxorubicin-induced nephrosis.

MeSH Terms
Animals Azepines/pharmacology Cells, Cultured Dose-Response Relationship, Drug Doxorubicin/pharmacology Drug Interactions Glomerular Mesangium/drug effects,immunology Interleukin-1/chemistry,metabolism Kidney Glomerulus/drug effects,immunology Kinetics Lipopolysaccharides/pharmacology Male Molecular Weight Platelet Activating Factor/antagonists & inhibitors Rats Rats, Sprague-Dawley Triazoles/pharmacology
Chemicals
Azepines Interleukin-1 Lipopolysaccharides Platelet Activating Factor Triazoles WEB 2086 Doxorubicin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bricio T
Department of Pathology, Hospital Ramon y Cajal, Madrid, Spain.
Molina A
Martin A
Mampaso F
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20 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1994-01-00
Pages
53-7
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1422285
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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