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PMID: 8132449 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

YscN, the putative energizer of the Yersinia Yop secretion machinery.

Journal of bacteriology ·Vol. 176 ·No. 6 ·1994-03-00 ·Pages 1561-9

Woestyn S, Allaoui A, Wattiau P, Cornelis GR

Abstract

Pathogenic yersiniae secrete a set of 11 antihost proteins called Yops. Yop secretion appears as the archetype of the type III secretion pathway. Several components of this machinery are encoded by the virA (lcrA) and virC (lcrC) loci of the 70-kb pYV plasmid. In this paper, we describe yscN, another gene involved in this pathway. It is the first gene of the virB locus. It encodes a 47.8-kDa protein similar to the catalytic subunits of F0F1 and related ATPases, as well as to products of other genes presumed to be involved in a type III secretion pathway. YscN contains the two consensus nucleotide-binding motifs (boxes A and B) described by Walker et al. (J. E. Walker, M. Saraste, M. J. Runswick, and N. J. Gay, EMBO J. 1:945-951, 1982). We engineered a pYV mutant encoding a modified YscN protein lacking box A. This mutant, impaired in Yop secretion, can be complemented in trans by a cloned yscN gene. We conclude that YscN is a component of the Yop secretion machinery using ATP. We hypothesize that it is either the energizer of this machinery or a part of it.

Related Genes
yscN
MeSH Terms
Adenosine Triphosphatases Amino Acid Sequence Bacterial Outer Membrane Proteins/metabolism Bacterial Proteins/genetics,physiology Base Sequence Carrier Proteins/genetics,physiology Cloning, Molecular Genes, Bacterial/physiology Molecular Sequence Data Mutagenesis, Site-Directed Plasmids/genetics Yersinia/genetics,physiology
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins Adenosine Triphosphatases YscN protein, Yersinia
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Woestyn S
Microbial Pathogenesis Unit, Université Catholique de Louvain, Brussels, Belgium.
Allaoui A
Wattiau P
Cornelis G R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.6.1561-1569.1994
Published
1994-03-00
Pages
1561-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205240
Subset
IM
Databases
GENBANK
U02499
External Links
PubMed source DOI: 10.1128/jb.176.6.1561-1569.1994 PMC Full Text View journal details
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