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PMID: 8132710 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

En bloc incorporation of coatomer subunits during the assembly of COP-coated vesicles.

The Journal of cell biology ·Vol. 124 ·No. 6 ·1994-03-00 ·Pages 883-92

Hara-Kuge S, Kuge O, Orci L, Amherdt M, Ravazzola M, Wieland FT, Rothman JE

Abstract

The cDNA encoding epsilon-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-epsilon-COP antibody showed that epsilon-COP exists in COP-coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant His6- tagged epsilon-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit.

Related Genes
&egr -COP
MeSH Terms
Amino Acid Sequence Animals Base Sequence CHO Cells Cattle Cloning, Molecular Coatomer Protein Cricetinae Golgi Apparatus/metabolism,ultrastructure Membrane Proteins/chemistry,genetics,metabolism Microscopy, Immunoelectron Molecular Sequence Data Organelles/metabolism,ultrastructure Rabbits Transfection
Chemicals
Coatomer Protein Membrane Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hara-Kuge S
Program of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, New York 10021.
Kuge O
Orci L
Amherdt M
Ravazzola M
Wieland F T
Rothman J E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.124.6.883
Published
1994-03-00
Pages
883-92
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119964
Subset
IM
Databases
GENBANK
X76980
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1083/jcb.124.6.883 PMC Full Text View journal details
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