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PMID: 8134379 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Hepatitis B virus X protein inhibits p53 sequence-specific DNA binding, transcriptional activity, and association with transcription factor ERCC3.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 6 ·1994-03-15 ·Pages 2230-4

Wang XW, Forrester K, Yeh H, Feitelson MA, Gu JR, Harris CC

Abstract

Chronic active hepatitis caused by infection with hepatitis B virus, a DNA virus, is a major risk factor for human hepatocellular carcinoma. Since the oncogenicity of several DNA viruses is dependent on the interaction of their viral oncoproteins with cellular tumor-suppressor gene products, we investigated the interaction between hepatitis B virus X protein (HBX) and human wild-type p53 protein. HBX complexes with the wild-type p53 protein and inhibits its sequence-specific DNA binding in vitro. HBX expression also inhibits p53-mediated transcriptional activation in vivo and the in vitro association of p53 and ERCC3, a general transcription factor involved in nucleotide excision repair. Therefore, HBX may affect a wide range of p53 functions and contribute to the molecular pathogenesis of human hepatocellular carcinoma.

MeSH Terms
Cell Line DNA/metabolism DNA-Binding Proteins/metabolism Drosophila Proteins Humans Mutation Protein Binding Trans-Activators/metabolism Transcription Factors/metabolism Transcriptional Activation Tumor Suppressor Protein p53/antagonists & inhibitors,genetics,metabolism Viral Regulatory and Accessory Proteins
Chemicals
DNA-Binding Proteins Drosophila Proteins Trans-Activators Transcription Factors Tumor Suppressor Protein p53 Viral Regulatory and Accessory Proteins hepatitis B virus X protein hay protein, Drosophila DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wang X W
Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Forrester K
Yeh H
Feitelson M A
Gu J R
Harris C C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.6.2230
Published
1994-03-15
Pages
2230-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43344
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.6.2230 PMC Full Text View journal details
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