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PMID: 8134384 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Temporal phosphorylation of the Drosophila period protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 6 ·1994-03-15 ·Pages 2260-4

Edery I, Zwiebel LJ, Dembinska ME, Rosbash M

Abstract

The period gene (per) is required for Drosophila melanogaster to manifest circadian (congruent to 24 hr) rhythms. We report here that per protein (PER) undergoes daily oscillations in apparent molecular mass as well as abundance. The mobility changes are largely or exclusively due to multiple phosphorylation events. The temporal profile of the classic short-period form of PER (PERS) is altered in a manner consistent with the mutant strain's behavioral phenotype. As changes in abundance and phosphorylation persist under constant environmental conditions, they reflect or contribute to a free-running rhythm. We suggest that the phosphorylation status of PER is an important determinant in the Drosophila clock's time-keeping mechanism.

MeSH Terms
Animals Blotting, Western Circadian Rhythm Drosophila Proteins Drosophila melanogaster/genetics,metabolism Mutation Nuclear Proteins/genetics,metabolism Period Circadian Proteins Phenotype Phosphorylation
Chemicals
Drosophila Proteins Nuclear Proteins PER protein, Drosophila Period Circadian Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Edery I
Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02254.
Zwiebel L J
Dembinska M E
Rosbash M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.6.2260
Published
1994-03-15
Pages
2260-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43350
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.6.2260 PMC Full Text View journal details
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