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PMID: 8139007 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human immunodeficiency virus type 1 Rev-responsive element RNA binds to host cell-specific proteins.

Journal of virology ·Vol. 68 ·No. 4 ·1994-04-00 ·Pages 2224-9

Shukla RR, Kimmel PL, Kumar A

Abstract

RNase protection-gel retention studies show human host cell-specific ribonucleoprotein complexes with human immunodeficiency virus type 1 Rev-responsive element (RRE) RNA. Nuclear proteins from rodent or murine cells appear to lack the ability to form these complexes. Human-mouse somatic cell hybrids retaining a single human chromosome, either 6 or 12, form the RRE-nuclear-protein complexes. One of the complexes requires the entire RRE RNA, while the other needs RRE RNA stem-loops 1 and 2 only. Two major proteins with molecular masses of 120 and 62 kDa specifically bind to RRE RNA. Rodent cells (CHO) either lack or contain small amounts of these RRE-binding proteins.

MeSH Terms
Animals Cross-Linking Reagents Genes, env HIV-1/metabolism HeLa Cells Humans Mice Models, Molecular Nucleic Acid Conformation Protein Binding RNA, Viral/metabolism RNA-Binding Proteins/metabolism Regulatory Sequences, Nucleic Acid Ribonucleoproteins/metabolism,radiation effects Species Specificity Ultraviolet Rays
Chemicals
Cross-Linking Reagents RNA, Viral RNA-Binding Proteins Ribonucleoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shukla R R
Department of Biochemistry and Molecular Biology, George Washington University Medical Center, Washington, D.C. 20037.
Kimmel P L
Kumar A
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42 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.4.2224-2229.1994
Published
1994-04-00
Pages
2224-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236698
Subset
IM
Grants
PHS HHS · A12531 · United States
NIDDK NIH HHS · NIDDK 1-RO-1-DK40811 · United States
External Links
PubMed source DOI: 10.1128/JVI.68.4.2224-2229.1994 PMC Full Text View journal details
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