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PMID: 8152413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 242 ·No. 6 ·1994-03-00 ·Pages 631-40

Yoshida S, Ohya Y, Nakano A, Anraku Y

Abstract

Loss of yeast protein kinase C function results in three distinct phenotypes: staurosporine sensitivity, cell lysis and blockage of cell cycle progression at the G2/M boundary. Genetic analysis of the PKC1/STT1 protein kinase C gene and its interactions with STT4, encoding an upstream phosphatidylinositol 4-kinase, and BCK1, encoding a downstream protein kinase, reveal that they form part of a single pathway. However, the BCK1-20 mutation (a gain-of-function mutation of BCK1) or overexpression of PKC1 cannot suppress all of the phenotypes caused by the loss of STT4 function, strongly suggesting the existence of a branch point between STT4 and PKC1. We also describe the MSS4 gene, a multicopy suppressor of the temperature-sensitive stt4-1 mutation. MSS4 is predicted to encode a hydrophilic protein of 779 amino acid residues and is essential for cell growth. Based on genetic and biochemical data, we suggest that MSS4 acts downstream of STT4, but in a pathway that does not involve PKC1.

Related Genes
BCK1 MSS4 PKC1 STT1 STT4
MeSH Terms
1-Phosphatidylinositol 4-Kinase Alkaloids/pharmacology Amino Acid Sequence Base Sequence Cell Cycle/genetics DNA, Fungal/genetics Drug Resistance, Microbial/genetics Genes, Fungal Genes, Suppressor Molecular Sequence Data Mutation Phenotype Phosphotransferases (Alcohol Group Acceptor)/genetics Protein Kinase C/genetics Protein Kinases/genetics Restriction Mapping Saccharomyces cerevisiae/drug effects,enzymology,genetics Saccharomyces cerevisiae Proteins Staurosporine Temperature
Chemicals
Alkaloids DNA, Fungal Saccharomyces cerevisiae Proteins Protein Kinases Phosphotransferases (Alcohol Group Acceptor) 1-Phosphatidylinositol 4-Kinase PIK1 protein, S cerevisiae Protein Kinase C Staurosporine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yoshida S
Department of Biology, Faculty of Science, University of Tokyo, Japan.
Ohya Y
Nakano A
Anraku Y
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38 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00283416
Published
1994-03-00
Pages
631-40
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
D13716
External Links
PubMed source DOI: 10.1007/BF00283416
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