Large subunit ribulosebisphosphate carboxylase messenger RNA from Euglena chloroplasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 3 ·1976-03-00 ·Pages 722-6

Sagher D, Grosfeld H, Edelman M

Abstract

RNA from wild-type Euglena, aplastidic mutant cells, and purified chloroplasts was chromatographed on oligo(dT)-cellulose at 4 degrees. The poly(A)-containing and poly(A)-lacking fractions from each were then tested in a cell-free protein-synthesizing system from wheat germ for their abilities to specifically stimulate synthesis of large subunit ribulosebisphosphate carboxylase [EC 4.1.1.39; 3-phospho-D-glycerate carboxy-lyase (dimerizing)]. The large subunit polypeptide (59,000 molecular weight) was identified in the in vitro reaction by two-dimensional electrophoresis involving isoelectric focusing and size filtration on polyacrylamide gels. Template activity for the large subunit was detected in wild-type cells but not in aplastidic mutant strains; it was highly enriched in the isolated chloroplasts. This messenger was present only in the poly(A)-lacking RNA fraction, where it constituted the most prominent template species of chloroplast RNA. The large subunit message was freed of considerable non-messenger RNA contamination and localized to the 10-20S fraction by sucrose gradient centrifugation.

MeSH Terms

Carboxy-Lyases/biosynthesis Chloroplasts/analysis Euglena gracilis Extrachromosomal Inheritance RNA, Messenger/isolation & purification Ribulose-Bisphosphate Carboxylase/biosynthesis

Chemicals

RNA, Messenger Carboxy-Lyases Ribulose-Bisphosphate Carboxylase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sagher D

Grosfeld H

Edelman M

References (24)

24 references, click to expand

Characterization of polyadenylate from the fungus Trichoderma viride.
J Bacteriol. 1975 Aug;123(2):765-7 PMID: 1171092
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
Isolation and characterization of Euglena gracilis cytoplasmic and chloroplast ribosomes and their ribosomal RNA components.
Biochim Biophys Acta. 1969 Oct 22;190(2):368-80 PMID: 4900576
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
STUDIES OF CHLOROPLAST DEVELOPMENT IN EUGLENA. XII. TWO TYPES OF SATELLITE DNA.
J Mol Biol. 1965 Apr;11:769-74 PMID: 14338786
Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336-40 PMID: 5288383
Polysome formation with tobacco mosaic virus RNA.
Proc Natl Acad Sci U S A. 1968 Apr;59(4):1243-50 PMID: 5240027
Major mRNA species from spinach chloroplasts do not contain poly(A).
Nature. 1975 Sep 4;257(5521):66-7 PMID: 1161008
Poly(A)-associated RNA in plants.
Biochim Biophys Acta. 1974 Apr 27;349(1):32-8 PMID: 11400435
A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331-5 PMID: 5288382
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4 PMID: 4525168
Distinct transcription products of ribosomal genes in two different tissues.
Nat New Biol. 1972 Jan 19;235(55):80-2 PMID: 4501345
An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321-5 PMID: 5288381
RNA metabolism in the HeLa cell nucleus.
J Mol Biol. 1966 May;17(1):117-30 PMID: 5961144
[Properties of Euglena gracilis ribosomes and ribosomal RNA].
Biochim Biophys Acta. 1970 Nov 12;224(1):144-54 PMID: 4923220
High biological activity in chloroplasts from Euglena gracilis prepared with a new gas pressure device.
FEBS Lett. 1970 Jun 1;8(3):164-166 PMID: 11947560
A one-step method for the isolation and determination of leaf ribulose-1,5-diphosphate carboxylase.
Anal Biochem. 1971 May;41(1):57-66 PMID: 5578554
Ribulose Diphosphate Carboxylase from Autotrophic Euglena gracilis.
Plant Physiol. 1975 Sep;56(3):345-50 PMID: 16659300
Polyadenylic acid sequences in E. coli messenger RNA.
Nature. 1975 Jul 10;256(5513):144-6 PMID: 1097936
Mitochondrial polyadenylic acid-containing RNA: localization and characterization.
J Mol Biol. 1973 Nov 5;80(3):379-91 PMID: 4762559
High resolution two-dimensional electrophoresis of proteins.
J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
Identification and partial characterization of multiple discrete polyadenylic acid containing RNA components coded for by HeLa cell mitochondrial DNA.
J Mol Biol. 1974 Sep 5;88(1):205-19 PMID: 4444037
Autotrophic CO2 assimilation and the evolution of ribulose diphosphate carboxylase.
Bacteriol Rev. 1973 Sep;37(3):289-319 PMID: 4357017
Protein synthesis in chloroplasts. V. Translation of messenger RNA for the large subunit of fraction I protein in a heterologous cell-free system.
J Mol Biol. 1975 Jan 5;91(1):67-77 PMID: 1102697

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.3.722

Published

1976-03-00

Pages

722-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC335990

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.3.722 PMC Full Text View journal details

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Large subunit ribulosebisphosphate carboxylase messenger RNA from Euglena chloroplasts.

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