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PMID: 8159721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Formation of in vivo complexes between the TAL1 and E2A polypeptides of leukemic T cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 8 ·1994-04-12 ·Pages 3181-5

Hsu HL, Wadman I, Baer R

Abstract

Tumor-specific activation of the TAL1 gene occurs in approximately 25% of patients with T-cell acute lymphoblastic leukemia (T-ALL). The TAL1 gene products possess a basic helix-loop-helix (bHLH) domain that interacts in vitro with the bHLH proteins (E12 and E47) encoded by the E2A locus. We have now applied two independent methods, the two-hybrid procedure and co-immunoprecipitation analysis, to demonstrate that TAL1 and E2A polypeptides also associate in vivo. These studies show that the bHLH domain of TAL1 selectively interacts with the bHLH domains of E12 and E47, but not with the Id1 helix-loop-helix protein. TAL1 does not self-associate to form homodimeric complexes, implying that the in vivo functions of TAL1 depend on heterologous interaction with other bHLH proteins such as E12 and E47. Co-immunoprecipitation analysis revealed the presence of endogenous TAL1/E2A complexes in Jurkat cells, a leukemic line derived from a T-ALL patient. Thus, the malignant properties of TAL1 may be due to obligate interaction with the E2A polypeptides.

MeSH Terms
Base Sequence Basic Helix-Loop-Helix Transcription Factors Cell Line DNA Primers/chemistry DNA-Binding Proteins/metabolism Helix-Loop-Helix Motifs Humans Leukemia-Lymphoma, Adult T-Cell/metabolism Molecular Sequence Data Protein Binding Proto-Oncogene Proteins T-Cell Acute Lymphocytic Leukemia Protein 1 TCF Transcription Factors Transcription Factor 7-Like 1 Protein Transcription Factors/metabolism
Chemicals
Basic Helix-Loop-Helix Transcription Factors DNA Primers DNA-Binding Proteins Proto-Oncogene Proteins T-Cell Acute Lymphocytic Leukemia Protein 1 TCF Transcription Factors TCF7L1 protein, human Transcription Factor 7-Like 1 Protein Transcription Factors TAL1 protein, human
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hsu H L
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
Wadman I
Baer R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.8.3181
Published
1994-04-12
Pages
3181-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43539
Subset
IM
Grants
NCI NIH HHS · CA46593 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.8.3181 PMC Full Text View journal details
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