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PMID: 8163943 Published · ppublish English Journal Article

Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease.

The Journal of experimental medicine ·Vol. 179 ·No. 5 ·1994-05-01 ·Pages 1653-58

Telford JL, Ghiara P, Dell'Orco M, Comanducci M, Burroni D, Bugnoli M, Tecce MF, Censini S, Covacci A, Xiang Z

Abstract

The gram negative, microaerophilic bacterium Helicobacter pylori colonizes the human gastric mucosa and establishes a chronic infection that is tightly associated with atrophic gastritis, peptic ulcer, and gastric carcinoma. Cloning of the H. pylori cytotoxin gene shows that the protein is synthesized as a 140-kD precursor that is processed to a 94-kD fully active toxin. Oral administration to mice of the purified 94-kD protein caused ulceration and gastric lesions that bear some similarities to the pathology observed in humans. The cloning of the cytotoxin gene and the development of a mouse model of human gastric disease will provide the basis for the understanding of H. pylori pathogenesis and the development of therapeutics and vaccines.

MeSH Terms
Amino Acid Sequence Animals Bacterial Proteins/genetics Bacterial Toxins/genetics Base Sequence Cloning, Molecular Cytotoxins/genetics DNA, Bacterial Disease Models, Animal Helicobacter Infections/microbiology Helicobacter pylori/genetics,pathogenicity Humans Immunoblotting Mice Mice, Inbred BALB C Molecular Sequence Data Peptide Fragments/genetics Stomach Diseases/microbiology Stomach Ulcer/microbiology
Chemicals
Bacterial Proteins Bacterial Toxins Cytotoxins DNA, Bacterial Peptide Fragments VacA protein, Helicobacter pylori
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Telford J L
Department of Molecular Biology, Immunobiological Research Institute Siena, Italy.
Ghiara P
Dell'Orco M
Comanducci M
Burroni D
Bugnoli M
Tecce M F
Censini S
Covacci A
Xiang Z
References (19)
19 references, click to expand
  1. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  2. Systemic and mucosal humoral responses to Helicobacter pylori in gastric cancer.
    Gut. 1993 Oct;34(10):1339-43 PMID: 8244098
  3. Campylobacter-like organisms and surface epithelium abnormalities in active, chronic gastritis in humans: an ultrastructural study.
    Ultrastruct Pathol. 1986;10(2):113-22 PMID: 3961927
  4. Expression and immunological properties of the five subunits of pertussis toxin.
    Infect Immun. 1987 Apr;55(4):963-7 PMID: 3549567
  5. Gastric Campylobacter-like organisms, gastritis, and peptic ulcer disease.
    Gastroenterology. 1987 Aug;93(2):371-83 PMID: 3297911
  6. Expression of diagnostic 31/32 kilodalton proteins of Schistosoma mansoni as fusions with bacteriophage MS2 polymerase.
    Mol Biochem Parasitol. 1988 Jan 15;27(2-3):233-9 PMID: 3125431
  7. Prevalence of Helicobacter pylori infection and histologic gastritis in asymptomatic persons.
    N Engl J Med. 1989 Dec 7;321(23):1562-6 PMID: 2586553
  8. Effect of urease on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin.
    Infect Immun. 1991 Apr;59(4):1264-70 PMID: 2004808
  9. Helicobacter pylori infection and the risk of gastric carcinoma.
    N Engl J Med. 1991 Oct 17;325(16):1127-31 PMID: 1891020
  10. Helicobacter pylori and gastroduodenal disease.
    Annu Rev Med. 1992;43:135-45 PMID: 1580578
  11. Purification and characterization of the vacuolating toxin from Helicobacter pylori.
    J Biol Chem. 1992 May 25;267(15):10570-5 PMID: 1587837
  12. Monoclonal antibodies against the native urease of Helicobacter pylori: synergistic inhibition of urease activity by monoclonal antibody combinations.
    Infect Immun. 1992 Nov;60(11):4826-31 PMID: 1383158
  13. Bafilomycin A1 inhibits Helicobacter pylori-induced vacuolization of HeLa cells.
    Mol Microbiol. 1993 Jan;7(2):323-7 PMID: 8446034
  14. Effects of ATPase inhibitors on the response of HeLa cells to Helicobacter pylori vacuolating toxin.
    Infect Immun. 1993 Apr;61(4):1427-31 PMID: 8454346
  15. Helicobacter pylori: host responses in peptic ulceration.
    Lancet. 1993 Apr 3;341(8849):900-1 PMID: 8096606
  16. The complete general secretory pathway in gram-negative bacteria.
    Microbiol Rev. 1993 Mar;57(1):50-108 PMID: 8096622
  17. Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production.
    Infect Immun. 1993 May;61(5):1799-809 PMID: 8478069
  18. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer.
    Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5791-5 PMID: 8516329
  19. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.179.5.1653
Published
1994-05-01
Pages
1653-58
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191472
Subset
IM
Databases
GENBANK
S72494
External Links
PubMed source DOI: 10.1084/jem.179.5.1653 PMC Full Text View journal details
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