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PMID: 8164682 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.

Molecular and cellular biology ·Vol. 14 ·No. 5 ·1994-05-00 ·Pages 3310-9

Dou S, Zeng X, Cortes P, Erdjument-Bromage H, Tempst P, Honjo T, Vales LD

Abstract

We have identified a cellular protein, RBP-2N, a presumed recombinase, as a repressor of transcription. Inhibition of transcription by RBP-2N was dependent on its DNA recognition site and was demonstrated in vitro and in vivo. This repression appears to be general, as transcription mediated by SP1 and Gal4/VP16 was inhibited by RBP-2N. The protein was purified to near homogeneity from human cells on the basis of its binding to a site present in the promoter of the adenovirus pIX gene. The DNA recognition sequence is 5'-TGGGAAAGAA, which is markedly different from the recombination signal sequence originally identified as the target site for this protein. The sequence of the purified protein is 97% identical with that published for the mouse RBP-2N protein. The reported homolog in Drosophila is Suppressor of Hairless. RBP-2N binding sites are present in a number of cellular and viral promoters, so RBP-2N may have a general role in transcriptional repression.

Related Genes
E1A E1B
MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Clone Cells DNA/metabolism DNA Nucleotidyltransferases/biosynthesis,isolation & purification,metabolism Drosophila/genetics,metabolism Genes, Immediate-Early HeLa Cells Humans Integrases Molecular Sequence Data Oligodeoxyribonucleotides/metabolism Peptide Fragments/chemistry,isolation & purification Plasmids Recombinases Recombination, Genetic Repressor Proteins/biosynthesis,isolation & purification,metabolism Transfection
Chemicals
Oligodeoxyribonucleotides Peptide Fragments RBP-2N protein, human Recombinases Repressor Proteins DNA DNA Nucleotidyltransferases Integrases integron integrase IntI1
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Dou S
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
Zeng X
Cortes P
Erdjument-Bromage H
Tempst P
Honjo T
Vales L D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.5.3310-3319.1994
Published
1994-05-00
Pages
3310-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358697
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.5.3310-3319.1994 PMC Full Text View journal details
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