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PMID: 816651 Published · ppublish English Journal Article

Transcription from complementary deoxyribonucleic acid strands in various sporogenic and asporogenic mutants of Bacillus subtilis. Hybridization-competition studies on ribonucleic acid synthesized in vivo by a thermosensitive sporulation mutant (ts-4).

European journal of biochemistry ·Vol. 63 ·No. 1 ·1976-03-16 ·Pages 53-63

Bonamy C, Manca de Nadra MC, Szulmajster J

Abstract

The present paper describes an investigation, at the transcription level, in a thermosensitive sporulation mutant of Bacillus subtilis (ts-4) grown at the permissive (30 degrees C) or restrictive (42 degrees C) temperature where sporulation capacity is respectively expressed or arrested. These studies were carried out by analysing the ribonucleic acid from vegetative and stationary phase cells (t3 cells) grown under both conditions, by hybridization-competition experiments.

MeSH Terms
Bacillus subtilis/metabolism Binding, Competitive Centrifugation, Density Gradient DNA, Bacterial/metabolism Mutation Nucleic Acid Hybridization RNA, Bacterial/biosynthesis Spores, Bacterial/metabolism Temperature Transcription, Genetic
Chemicals
DNA, Bacterial RNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bonamy C
Manca de Nadra M C
Szulmajster J
Article Info
Journal
European journal of biochemistry
Abbr.
Eur J Biochem
ISSN
0014-2956
Published
1976-03-16
Pages
53-63
Language
English
Region
England
NLM ID
0107600
Subset
IM
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