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PMID: 8166641 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Angiotensin II stimulates phosphorylation of high-molecular-mass cytosolic phospholipase A2 in vascular smooth-muscle cells.

The Biochemical journal ·Vol. 299 ( Pt 1) ·1994-04-01 ·Pages 197-201

Rao GN, Lassègue B, Alexander RW, Griendling KK

Abstract

Phospholipase A2 (PLA2) may be one of the major components involved in cell signalling and proliferation, as suggested by recent studies. In this paper we show that the potent vasoconstrictor and hypertrophic agent angiotensin II (AngII) activates cytosolic PLA2 (cPLA2) in vascular smooth-muscle cells. AngII induced a rapid time-dependent release of [3H]arachidonic acid from prelabelled cells that was inhibited by mepacrine, a PLA2 inhibitor. AngII treatment of intact cells also activated a cPLA2, as measured in cell-free extracts by the release of radiolabelled arachidonic acid from exogenously added 1-stearoyl-2-[1-14C]arachidonoyl phosphatidylcholine. This AngII-stimulated cPLA2 activity was also significantly inhibited by mepacrine. AngII induced a rapid and time-dependent increase in cPLA2 phosphorylation. Protein kinase C (PKC) depletion inhibited both AngII-induced [3H]arachidonic acid release and cPLA2 phosphorylation. Together, these results suggest strongly that (1) AngII phosphorylates and activates cPLA2 in a PKC-dependent manner, and that (2) cPLA2 mediates the AngII-induced [3H]arachidonic acid release in vascular smooth-muscle cells.

MeSH Terms
Angiotensin II/pharmacology Animals Arachidonic Acid/metabolism Cells, Cultured Cytosol/enzymology Enzyme Activation Kinetics Male Molecular Weight Muscle, Smooth, Vascular/cytology,drug effects,enzymology Phospholipases A/metabolism Phospholipases A2 Phosphorylation/drug effects Rats Rats, Sprague-Dawley
Chemicals
Angiotensin II Arachidonic Acid Phospholipases A Phospholipases A2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rao G N
Division of Cardiology, Emory University School of Medicine, Atlanta, GA 30322.
Lassègue B
Alexander R W
Griendling K K
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2990197
Published
1994-04-01
Pages
197-201
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1138041
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2990197 PMC Full Text View journal details
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