Human lysosomal alpha-mannosidase: isolation and nucleotide sequence of the full-length cDNA.

Biochemical and biophysical research communications ·Vol. 200 ·No. 1 ·1994-04-15 ·Pages 239-45

Nebes VL, Schmidt MC

Abstract

The amino acid sequence of the human lysosomal alpha-mannosidase precursor has been deduced by PCR mediated cloning and sequencing of the cDNA. The protein has 961 amino acids and a molecular weight of 107,644 Daltons. The amino acid sequence shows 38% identity to the Dictyostelium discoideum lysosomal alpha-mannosidase. The cDNA maps proximal to the centromere on chromosome 19q, the same locus as the MANB gene. Our results provide valuable information for the study of the lysosomal storage disease alpha-mannosidosis, an inherited disorder caused by mutations in the MANB gene which encodes the human lysosomal alpha-mannosidase.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Chromosome Mapping Chromosomes, Human, Pair 19 Cloning, Molecular Consensus Sequence DNA Primers DNA, Complementary/analysis Dictyostelium/enzymology,genetics Gene Library HeLa Cells Humans Lysosomes/enzymology Mannosidases/chemistry,genetics Mice Molecular Sequence Data Molecular Weight Polymerase Chain Reaction/methods Rats Sequence Homology, Amino Acid alpha-Mannosidase

Chemicals

DNA Primers DNA, Complementary Mannosidases alpha-Mannosidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Nebes V L

Dept. of Ophthalmology, Allegheny-Singer Research Institute, Pittsburgh, PA 15212.

Schmidt M C

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1006/bbrc.1994.1440

Published

1994-04-15

Pages

239-45

Language

English

Region

United States

NLM ID

0372516

Subset

Databases

GENBANK

U05572

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1006/bbrc.1994.1440 View journal details

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Human lysosomal alpha-mannosidase: isolation and nucleotide sequence of the full-length cDNA.

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