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PMID: 8168924 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular analysis of urease genes from a newly identified uncultured species of Helicobacter.

Infection and immunity ·Vol. 62 ·No. 5 ·1994-05-00 ·Pages 1631-8

Solnick JV, O'Rourke J, Lee A, Tompkins LS

Abstract

"Gastrospirillum hominis" is an uncultured gastric spiral bacterium that has recently been shown by 16S rDNA sequence analysis to be a newly recognized species of Helicobacter that infects humans, and it has been provisionally designated "Helicobacter heilmannii." We used PCR to directly amplify the urease structural genes of "H. heilmannii" from infected gastric tissue. DNA sequence analysis identified two open reading frames, ureA and ureB, which code for polypeptides with predicted molecular weights of 25,729 and 61,831, respectively. The urease subunit genes from "H. heilmannii" were cloned and expressed in Escherichia coli. Western blot (immunoblot) analysis showed that antiserum directed against the ureA and ureB gene products from H. pylori was cross-reactive with the corresponding polypeptides from "H. heilmannii." Analysis of the derived amino acid sequences of "H. heilmannii" UreA and UreB demonstrated that "H. heilmannii" urease is more highly related to the urease from H. felis (found in the stomachs of cats and dogs) than to the urease from H. pylori. These data are consistent with 16S rDNA sequence analysis and suggest that "H. heilmannii" is phylogenetically most closely related to H. felis.

Related Genes
ureA ureB
MeSH Terms
Amino Acid Sequence Base Composition Base Sequence Genes, Bacterial Helicobacter/enzymology,genetics Molecular Sequence Data Urease/chemistry,genetics
Chemicals
Urease
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Solnick J V
Department of Medicine, Stanford University School of Medicine, California 94305.
O'Rourke J
Lee A
Tompkins L S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.62.5.1631-1638.1994
Published
1994-05-00
Pages
1631-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC186371
Subset
IM
Grants
NIAID NIH HHS · AI-23796 · United States
Databases
GENBANK
L25079
External Links
PubMed source DOI: 10.1128/iai.62.5.1631-1638.1994 PMC Full Text View journal details
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