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PMID: 817846 Published · ppublish fre English Abstract Journal Article

[A genetic transfer system in a cyanophyte of the genus Aphanocapsa].

Mise en évidence d'un système de transfert génétique chez une cyanophycée du genre Aphanocapsa

Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles ·Vol. 282 ·No. 8 ·1976-02-23 ·Pages 795-7

Astier C, Espardellier F

Abstract

The possibility of a genetic transfer, through a process of transformation, has been shown in a unicellular, facultative chemoheterotroph blue-green Algae, Aphanocapsa 6714. Similarly to the process described with Anacystis nidulans, the recipient strain becomes competent when the growth reaches its second, slower, exponential phase. The genetic marker conferring resistance to p-F-phenylalanine has been transferred with a frequency 20 to 100 times higher than that of the spontaneous mutations. The efficiency of transformation decreases by a factor of 70% when the transforming extract, which contains both DNA and RNA, is pretreated with DNase. This transfer process is compared to that observed with A. nidulans by several authors.

MeSH Terms
Cyanobacteria/growth & development,physiology DNA/metabolism Genes Transformation, Genetic
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Astier C
Espardellier F
Article Info
Journal
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles
Abbr.
C R Acad Hebd Seances Acad Sci D
Published
1976-02-23
Pages
795-7
Language
fre
Region
France
NLM ID
7501107
Subset
IM
External Links
PubMed source
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