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PMID: 8187764 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

UV light-induced DNA synthesis arrest in HeLa cells is associated with changes in phosphorylation of human single-stranded DNA-binding protein.

The EMBO journal ·Vol. 13 ·No. 9 ·1994-05-01 ·Pages 2114-23

Carty MP, Zernik-Kobak M, McGrath S, Dixon K

Abstract

We show that DNA replication activity in extracts of human HeLa cells decreases following UV irradiation. Alterations in replication activity in vitro parallel the UV-induced block in cell cycle progression of these cells in culture. UV irradiation also induces specific changes in the pattern of phosphorylation of the 34 kDa subunit of a DNA replication protein, human single-stranded DNA-binding protein (hSSB). The appearance of a hyperphosphorylated form of hSSB correlates with reduced in vitro DNA replication activity in extracts of UV-irradiated cells. Replication activity can be restored to these extracts in vitro by addition of purified hSSB. These results suggest that UV-induced DNA synthesis arrest may be mediated in part through phosphorylation-related alterations in the activity of hSSB, an essential component of the DNA replication apparatus.

MeSH Terms
Cell Cycle DNA Replication/radiation effects DNA-Binding Proteins/metabolism HeLa Cells Humans Phosphorylation/radiation effects Replication Protein A Ultraviolet Rays
Chemicals
DNA-Binding Proteins RPA1 protein, human Replication Protein A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Carty M P
Department of Environmental Health, College of Medicine, University of Cincinnati, OH 45267-0056.
Zernik-Kobak M
McGrath S
Dixon K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-05-01
Pages
2114-23
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395063
Subset
IM
Grants
NIEHS NIH HHS · ES04908 · United States
NIEHS NIH HHS · ES05400 · United States
NIEHS NIH HHS · ES06096 · United States
External Links
PubMed source PMC Full Text View journal details
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