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PMID: 8187775 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

ADP-dependent phosphorylation regulates RNA-binding in vitro: implications in light-modulated translation.

The EMBO journal ·Vol. 13 ·No. 9 ·1994-05-01 ·Pages 2227-35

Danon A, Mayfield SP

Abstract

Light-regulated translation of chloroplastic mRNAs in the green alga Chlamydomonas reinhardtii requires nuclear encoded factors that interact with the 5'-untranslated region (5'-UTR) of specific mRNAs to enhance their translation. We have previously identified and characterized a set of proteins that bind specifically to the 5'-UTR of the chloroplastic psbA mRNA. Accumulation of these proteins is similar in dark- and light-grown cells, whereas their binding activity is enhanced during growth in the light. We have identified a serine/threonine protein phosphotransferase, associated with the psbA mRNA-binding complex, that utilizes the beta-phosphate of ADP to phosphorylate and inactivate psbA mRNA-binding in vitro. The inactivation of mRNA-binding in vitro is initiated at high ADP levels, levels that are attained in vivo only in dark-grown chloroplasts. These data suggest that the translation of psbA mRNA is attenuated by phosphorylation of the mRNA-binding protein complex in response to a rise in the stromal concentration of ADP upon transfer of cells to dark.

MeSH Terms
Adenosine Diphosphate/metabolism Adenosine Triphosphate/antagonists & inhibitors,metabolism Animals Bacterial Proteins/metabolism Chlamydomonas reinhardtii/genetics,growth & development,metabolism Chloroplasts/metabolism Introns Light Phosphorylation Photosynthetic Reaction Center Complex Proteins/metabolism Photosystem II Protein Complex Plant Proteins/metabolism Protein Biosynthesis/radiation effects Protein Kinases/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/metabolism
Chemicals
Bacterial Proteins Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins RNA, Messenger RNA-Binding Proteins Adenosine Diphosphate Adenosine Triphosphate Protein Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Danon A
Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037.
Mayfield S P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-05-01
Pages
2227-35
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395078
Subset
IM
Grants
NIGMS NIH HHS · GM41353 · United States
External Links
PubMed source PMC Full Text View journal details
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