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PMID: 8189203 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Hypotonicity activates a native chloride current in Xenopus oocytes.

The Journal of general physiology ·Vol. 103 ·No. 2 ·1994-02-00 ·Pages 153-79

Ackerman MJ, Wickman KD, Clapham DE

Abstract

Xenopus oocytes are frequently utilized for in vivo expression of cellular proteins, especially ion channel proteins. A thorough understanding of the endogenous conductances and their regulation is paramount for proper characterization of expressed channel proteins. Here we detail a novel chloride current (ICl.swell) responsive to hypotonicity in Xenopus oocytes using the two-electrode voltage clamp technique. Reducing the extracellular osmolarity by 50% elicited a calcium-independent chloride current having an anion conductivity sequence identical with swelling-induced chloride currents observed in epithelial cells. The hypotonicity-activated current was blocked by chloride channel blockers, trivalent lanthanides, and nucleotides. G-protein, cAMP-PKA, and arachidonic acid signaling cascades were not involved in ICl.swell activation. ICl.swell is distinct from both stretch-activated nonselective cation channels and the calcium-activated chloride current in oocytes and may play a critical role in volume regulation in Xenopus oocytes.

MeSH Terms
Animals Biotransformation/drug effects Chloride Channels/drug effects,metabolism Egtazic Acid/analogs & derivatives,pharmacology Electrophysiology Female Hydrogen-Ion Concentration Kinetics Membrane Potentials/physiology Metals, Rare Earth/pharmacology Nucleotides/pharmacology Oocytes/metabolism Osmolar Concentration Xenopus laevis
Chemicals
Chloride Channels Metals, Rare Earth Nucleotides 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester Egtazic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ackerman M J
Department of Pharmacology, Mayo Foundation, Rochester, Minnesota 55905.
Wickman K D
Clapham D E
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.103.2.153
Published
1994-02-00
Pages
153-79
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2216836
Subset
IM
Grants
PHS HHS · 44025 · United States
External Links
PubMed source DOI: 10.1085/jgp.103.2.153 PMC Full Text View journal details
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