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PMID: 8189522 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cellular protein modulates effects of human immunodeficiency virus type 1 Rev.

Journal of virology ·Vol. 68 ·No. 6 ·1994-06-00 ·Pages 3850-6

Luo Y, Yu H, Peterlin BM

Abstract

Replication of human immunodeficiency virus type 1 requires expression of the viral trans activator Rev. Rev binds to a highly structured RNA, the Rev response element, which is present in singly spliced and unspliced genomic viral RNAs. Although Rev helps to transport these transcripts from the nucleus to the cytoplasm, the mechanism(s) involved is not fully understood. Using the yeast two-hybrid system, we isolated a murine protein (YL2) that interacts with the basic domain of Rev, which is essential for the function of Rev in vivo and for the inhibitory splicing activity of Rev in vitro. YL2 has 92% identity to a human 32-kDa protein (p32), which copurifies with alternative splicing factor SF2/ASF. Furthermore, we found that whereas expression of YL2 greatly potentiated the activity of Rev, antisense YL2 transcripts blocked the effects of Rev in mammalian cells. YL2 also increased the activities of Rex on the Rex response element and of hybrid Rev proteins fused to Tat and the coat protein of bacteriophage MS2 on their respective RNAs. Thus, YL2 or p32 is a cellular protein that modulates the function of human immunodeficiency virus type 1 Rev.

MeSH Terms
Amino Acid Sequence Animals Carrier Proteins Cell Line Gene Products, rev/genetics,physiology HIV-1/genetics,physiology Humans Hyaluronan Receptors Mice Mitochondrial Proteins Molecular Sequence Data Proteins/genetics,metabolism RNA Splicing RNA, Viral/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Virus Replication/genetics,physiology rev Gene Products, Human Immunodeficiency Virus
Chemicals
C1QBP protein, human C1qbp protein, mouse Carrier Proteins Gene Products, rev Hyaluronan Receptors Mitochondrial Proteins Proteins RNA, Viral rev Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Luo Y
Howard Hughes Medical Institute, San Francisco, California.
Yu H
Peterlin B M
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52 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.6.3850-3856.1994
Published
1994-06-00
Pages
3850-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236890
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.6.3850-3856.1994 PMC Full Text View journal details
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