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PMID: 8192465 Published · ppublish English Comparative Study Journal Article

In vitro studies on stability and development of metronidazole resistance in Helicobacter pylori.

Antimicrobial agents and chemotherapy ·Vol. 38 ·No. 2 ·1994-02-00 ·Pages 360-2

van Zwet AA, Thijs JC, Schievink-de Vries W, Schiphuis J, Snijder JA

Abstract

Seventy isolates of Helicobacter pylori from antral biopsy samples were tested for their susceptibilities to metronidazole by agar dilution. Seven (10%) of these clinical isolates appeared to be resistant to metronidazole. Sixty-three strains were susceptible. In 42 (67%) of the 63 susceptible isolates, resistant isolates were obtained by serial passage on plates containing subinhibitory concentrations of metronidazole. In 10 of these 42 strains, the acquired resistance appeared to be unstable. The difference between the stability of resistance that occurred after one or two passages and the stability of resistance that occurred after three passages was statistically significant (P < 0.006). Primary resistance in clinical isolates was a stable phenomenon. Whether the resistance that emerges during therapy in patients is stable or unstable needs to be established.

MeSH Terms
Drug Resistance, Microbial Drug Stability Helicobacter pylori/drug effects,physiology Humans Metronidazole/pharmacology Microbial Sensitivity Tests
Chemicals
Metronidazole
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van Zwet A A
Regional Public Health Laboratory, Groningen, The Netherlands.
Thijs J C
Schievink-de Vries W
Schiphuis J
Snijder J A
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.38.2.360
Published
1994-02-00
Pages
360-2
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC284456
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.38.2.360 PMC Full Text View journal details
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