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PMID: 8193299 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.).

Plant molecular biology ·Vol. 24 ·No. 5 ·1994-03-00 ·Pages 743-55

Sparvoli F, Martin C, Scienza A, Gavazzi G, Tonelli C

Abstract

Genes involved in flavonoid and stilbene biosynthesis were isolated from grape (Vitis vinifera L.). Clones coding for phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX) and UDP glucose:flavonoid 3-O-glucosyl transferase (UFGT), were isolated by screening a cDNA library, obtained from mRNA from seedlings grown in light for 48 h using snapdragon (Antirrhinum majus) and maize heterologous probes. A cDNA clone coding for stilbene synthase (StSy) was isolated by probing the library with a specific oligonucleotide. These clones were sequenced and when the putative products were compared to the published amino acid sequence for corresponding enzymes, the percentages of similarity ranged from 65% (UFGT) to 90% (CHS and PAL). The analysis of the genomic organization and expression of these genes in response to light shows that PAL and StSy genes belong to large multigene families, while the others are present in one to four copies per haploid genome. The steady-state level of mRNAs encoded by the flavonoid biosynthetic genes as determined in young seedlings is coordinately induced by light, except for PAL and StSy, which appear to be constitutively expressed.

MeSH Terms
Anthocyanins/metabolism Cloning, Molecular Flavonoids/metabolism Fruit/enzymology,genetics,growth & development Gene Expression Regulation/radiation effects Genes, Plant/genetics Genome Light Molecular Sequence Data Multigene Family/genetics Sequence Analysis Sequence Homology Stilbenes/metabolism Tissue Distribution
Chemicals
Anthocyanins Flavonoids Stilbenes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sparvoli F
Dipartimento di Genetica e di Biologia dei Microorganismi, Università degli Studi di Milano, Italia.
Martin C
Scienza A
Gavazzi G
Tonelli C
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00029856
Published
1994-03-00
Pages
743-55
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
X75963, X75964, X75965, X75966, X75967, X75968, X75969, X76892
External Links
PubMed source DOI: 10.1007/BF00029856 View journal details
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