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PMID: 8194200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Different sialyltransferase activities in human colorectal carcinoma cells from surgical specimens detected by specific glycoprotein and glycolipid acceptors.

Clinical & experimental metastasis ·Vol. 12 ·No. 3 ·1994-05-00 ·Pages 245-54

Kemmner W, Krück D, Schlag P

Abstract

The amount and type of sialylation of tumor cell membranes depends on the activity of a number of different sialyltransferase enzymes. For the detection of specific activities in human colorectal carcinoma tissue several glycoprotein and glycolipid acceptors were used: desialylated fetuin, alpha 1-acid glycoprotein, beta 2-glycoprotein I, ovine submaxillaris mucin, and the gangliosides GM1, GM2, GM3 and GD1a. Because of their possible relevance for metastasis, precursors of Le(a) and Le(x) antigens, too, were employed, namely neoglycolipids produced by coupling LcOse4 or NeoLcOse4 oligosaccharides to L-alpha-phosphatidyl-ethanol-amine-dipalmitoyl. Our data indicate that human colorectal tumor tissue contains two highly active sialyltransferase enzymes, which are only weakly expressed in normal mucosa. These are a N-glycan-specific alpha 2,6-sialyltransferase, which was significantly increased in metastasizing tumors, and a Gal beta 1,3Gal-NAc-specific sialyltransferase, which was increased in tumors of early stages. A shift to enhanced alpha 2,6-sialylation of membrane glycoproteins during carcinogenesis was demonstrated by lectin ELISA analysis of magneto-bead separated tumor cells. Quantitative determination of specific sialyltransferase activities may be a sensitive tool for detection and monitoring of colon carcinoma.

MeSH Terms
Carcinoma/enzymology Colorectal Neoplasms/enzymology Gangliosides/metabolism Glycolipids/metabolism Glycoproteins/metabolism Humans Sialyltransferases/metabolism
Chemicals
Gangliosides Glycolipids Glycoproteins Sialyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kemmner W
Institut für Biochemie II, Universität Heidelberg, Germany.
Krück D
Schlag P
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Article Info
Journal
Clinical & experimental metastasis
Abbr.
Clin Exp Metastasis
ISSN
0262-0898
DOI
10.1007/BF01753893
Published
1994-05-00
Pages
245-54
Language
English
Region
Netherlands
NLM ID
8409970
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01753893 View journal details
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