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PMID: 8196664 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The transforming growth factor beta type II receptor can replace the activin type II receptor in inducing mesoderm.

Molecular and cellular biology ·Vol. 14 ·No. 6 ·1994-06-00 ·Pages 4280-5

Bhushan A, Lin HY, Lodish HF, Kintner CR

Abstract

The type II receptors for the polypeptide growth factors transforming growth factor beta (TGF-beta) and activin belong to a new family of predicted serine/threonine protein kinases. In Xenopus embryos, the biological effects of activin and TGF-beta 1 are strikingly different; activin induces a full range of mesodermal cell types in the animal cap assay, while TGF-beta 1 has no effects, presumably because of the lack of functional TGF-beta receptors. In order to assess the biological activities of exogenously added TGF-beta 1, RNA encoding the TGF-beta type II receptor was introduced into Xenopus embryos. In animal caps from these embryos, TGF-beta 1 and activin show similar potencies for induction of mesoderm-specific mRNAs, and both elicit the same types of mesodermal tissues. In addition, the response of animal caps to TGF-beta 1, as well as to activin, is blocked by a dominant inhibitory ras mutant, p21(Asn-17)Ha-ras. These results indicate that the activin and TGF-beta type II receptors can couple to similar signalling pathways and that the biological specificities of these growth factors lie in their different ligand-binding domains and in different competences of the responding cells.

MeSH Terms
Activin Receptors Activins Animals Embryo, Nonmammalian/cytology,physiology Inhibins/pharmacology Mesoderm/cytology,drug effects,physiology Protein Serine-Threonine Kinases/metabolism Proto-Oncogene Proteins p21(ras)/metabolism Receptors, Growth Factor/biosynthesis,physiology Receptors, Transforming Growth Factor beta/biosynthesis,physiology Signal Transduction Transforming Growth Factor beta/pharmacology Xenopus laevis/embryology
Chemicals
Receptors, Growth Factor Receptors, Transforming Growth Factor beta Transforming Growth Factor beta Activins Inhibins Protein Serine-Threonine Kinases Activin Receptors Proto-Oncogene Proteins p21(ras)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bhushan A
Molecular Neurobiology Laboratory, Salk Institute, La Jolla, California 92037.
Lin H Y
Lodish H F
Kintner C R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.6.4280-4285.1994
Published
1994-06-00
Pages
4280-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358794
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.6.4280-4285.1994 PMC Full Text View journal details
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